Entecavir up-regulates dendritic cell function in patients with chronic hepatitis B
Entecavir up-regulates dendritic cell function in patients with chronic hepatitis B
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DOI：
10.3748/wjg.14.1617
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发表时间：
2008-03-14
影响因子：
4.3
通讯作者：
Qi, Yuan-Ming
中科院分区：
文献类型：
--
作者：
Lu, Gao-Feng;Tang, Fu-Ai;Qi, Yuan-Ming
目的：目的探讨恩替卡韦（ETV）对慢性B肝炎（CHB）患者树突状细胞（DC）功能的影响。将DC与添加有胎牛血清、IL-4、粒细胞-巨噬细胞集落刺激因子（GM-CSF）的RPMI-1640培养基一起孵育。在第四天用或不用ETV处理DC。流式细胞术检测细胞表面分子CD 1a、CD 80、CD 83和HLA-DR。采用酶联免疫吸附试验（ELISA）检测细胞培养上清中IL-6和IL-12的浓度。观察所产生的DC刺激淋巴细胞增殖的能力。与CHB对照组相比，（29.07 +/- 3.20 vs 26.85 +/- 2.80），CD 83 ETV治疗组CD 80（28.00 ± 2.76 vs 25.75 ± 2.51）和HLA-DR（41.96 ± 3.81 vs 32.20 ± 3.04）高于对照组（P < 0.05）。ETV处理组分泌的IL-12（157.60 +/- 26.85 pg/mL对132.60 +/- 22.00 pg/mL（P < 0.05））显著高于CHB对照组，培养上清液中的IL-6水平（83.05 +/- 13.88 pg/mL对93.60 +/- 13.61 pg/mL，P < 0.05）低于CHB对照组。ETV治疗组DCs刺激同种异体淋巴细胞增殖的能力较CHB对照组增强（1.53 ± 0.09vs1.42 ± 0.08，P < 0.05）。(C)2008年WJG。All rights reserved.
AIM: To investigate the in vitro effect of entecavir (ETV) on the function of dendritic cells (DCs) derived from chronic hepatitis B (CHB) patients.METHODS: Mononuclear cells were isolated from peripheral blood of patients with CHB. DCs were incubated with RPMI-1640 medium supplemented with fetal bovine serum, IL-4, granulocyte-macrophage colony-stimulating factor (GM-CSF). DCs were treated with or without ETV on the fourth day. Cell surface molecules, including CD1a, CD80, CD83 and HLA-DR, were assessed by flow cytometry. Concentrations of IL-6 and IL-12 in the supernatant were assayed by enzyme-linked immunosorbent assay (ELISA). The ability of the generated DCs to stimulate lymphocyte proliferation was observed.RESULTS: Compared with CHB control group, the expression levels of CD1a (29.07 +/- 3.20 vs 26.85 +/- 2.80), CD83 (25.66 +/- 3.19 vs 23.21 +/- 3.10), CD80 (28.00 +/- 2.76 vs 25.75 +/- 2.51) and HLA-DR (41.96 +/- 3.81 vs 32.20 +/- 3.04) in ETV-treated group were higher (P < 0.05). ETV-treated group secreted significantly more IL-12 (157.60 +/- 26.85 pg/mL vs 132.60 +/- 22.00 pg/mL (P < 0.05) and had a lower level of IL-6 in the culture supernatant (83.05 +/- 13.88 pg/mL vs 93.60 +/- 13.61 pg/mL, P < 0.05) than CHB control group. The ability of DCs to stimulate the proliferation of allogeneic lymphocytes was increased in ETV-treated group compared with CHB control group (1.53 +/- 0.09 vs 1.42 +/- 0.08, P < 0.05).CONCLUSION: Entecavir can enhance the biological activity of DCs derived from CHB patients. (C) 2008 WJG. All rights reserved.