In vivo neuronal tract tracing using manganese-enhanced magnetic resonance imaging
In vivo neuronal tract tracing using manganese-enhanced magnetic resonance imaging
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DOI：
10.1002/mrm.1910400515
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发表时间：
1998-11-01
影响因子：
3.3
通讯作者：
Koretsky, AP
中科院分区：
文献类型：
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作者：
Pautler, RG;Silva, AC;Koretsky, AP
开发有效的成像技术来追踪神经元连接将是非常有用的。锰离子（Mn ~（2+））是磁共振成像（MRI）的优良T-1造影剂。四份利用放射性Mn ~（2+）在鱼和大鼠脑中的研究报告表明，Mn ~（2+）可用于追踪神经元连接。因此，这项工作的目的是确定Mn 2+是否可以用作体内MRI神经束示踪剂。结果表明，局部施用MnCl 2溶液到小鼠的鼻孔以及经由玻璃体内注射到视网膜神经节细胞导致对比度沿各自的路径的增强沿着。因此，将Mn 2+应用于神经元允许使用MRI来可视化神经元连接。
Development of efficient imaging techniques to trace neuronal connections would be very useful. Manganese ion (Mn2+) is an excellent T-1 contrast agent for magnetic resonance imaging (MRI). Four reports utilizing radioactive Mn2+ in fish and rat brain indicate that Mn2+ may be useful for tracing neuronal connections. Therefore, the purpose of this work was to determine if Mn2+ can be used as an in vivo MRI neuronal tract tracer. The results indicate that topical administration of MnCl2 solution to the naris of mice as well as to the retinal ganglion cells via intravitreal injection leads to enhancement of contrast along the respective pathways. Therefore, application of Mn2+ to neurons allows the use of MRI to visualize neuronal connections.