Effects of TGF-beta 1 on interleukin profile of human dental pulp and odontoblasts.
Effects of TGF-beta 1 on interleukin profile of human dental pulp and odontoblasts.
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发表时间：
2007
期刊：
影响因子：
3.8
通讯作者：
V. Pääkkönen;J. Vuoristo;T. Salo;L. Tjäderhane
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作者：
V. Pääkkönen;J. Vuoristo;T. Salo;L. Tjäderhane
转化生长因子-β 1（Transforming growth factor-beta 1，TGF-β 1）是牙本质-牙髓复合体中研究最广泛的生长因子，对牙髓反应和愈合具有多效性作用。我们的主要目的是分析牙髓组织和成牙本质细胞的表达谱，以及TGF-β 1对培养的人牙髓和成牙本质细胞中这些谱的影响，特别是对抗炎和促炎细胞因子的影响。为此目的，牙髓和成牙本质细胞培养不同的时间段，和微阵列进行培养和天然样品。在细胞因子中，各种白细胞介素（IL）通过RT-PCR确认，并且在+/-TGF-β 1处理的牙髓中也通过抗体阵列确认。在天然牙髓中检测到促炎性IL-7、-12 α和-16 mRNA。TGF-β 1处理后，促炎性IL-1 α、-1 β、-6和-8的表达水平明显诱导，而抗炎性细胞因子未诱导。在所有分析的牙髓白细胞介素中，IL-6和IL-8在条件牙髓组织培养基中以最高水平存在。在天然成牙本质细胞中检测到促炎性IL-6和-7 mRNA，在培养的成牙本质细胞中，促炎性IL-8 mRNA在TGF-β 1处理后显示出超过20倍的瞬时诱导。我们的研究结果表明，TGF-β 1是一个有效的调节剂的促炎反应和防御反应的牙本质牙髓复合体。
Transforming growth factor-beta 1 (TGF-beta1) is the most extensively studied growth factor in dentin-pulp complex, with pleiotropic effects on pulp response and healing. Our main objective was to analyze the expression profile of pulp tissue and odontoblasts, and the effects of TGF-beta1 on these profiles in cultured human pulp and odontoblasts with a specific interest in the anti- and pro-inflammatory cytokines. For that purpose, pulps and odontoblasts were cultured for different time periods, and microarray was performed to both cultured and native samples. Of cytokines, various interleukins (IL) were confirmed by RT-PCR, and in +/- TGF-beta1 treated pulps also by antibody array. Pro-inflammatory IL-7, -12alpha and -16 mRNAs were detected in native pulp. The expression levels of pro-inflammatory IL-1alpha, -1beta, -6 and -8 were clearly induced after TGF-beta1 treatment, while no anti-inflammatory cytokines were induced. Of all pulpal interleukins analyzed IL-6 and -8 were present at the highest levels in conditioned pulp tissue media. In native odontoblasts pro-inflammatory IL-6 and -7 mRNAs were detected, and in cultured odontoblasts pro-inflammatory IL-8 mRNA showed over 20-fold transient induction after TGF-beta1 treatment. Our results demonstrate that TGF-beta1 is a potent regulator of pro-inflammatory responses and defensive reactions in dentin-pulp complex.