Cloning, structural organization, and transcriptional activity of the rat vitamin K-dependent gamma-glutamyl carboxylase gene.
Cloning, structural organization, and transcriptional activity of the rat vitamin K-dependent gamma-glutamyl carboxylase gene.
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大鼠维生素 K 依赖性 γ-谷氨酰羧化酶基因的克隆、结构组织和转录活性。
DOI：
10.1006/bbrc.1998.8987
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Roth,DA
中科院分区：
文献类型：
--
作者：
Romero,EE;Deo,R;Velazquez-Estades,LJ;Roth,DA
从大鼠肝脏基因组文库中克隆了依赖维生素K的γ谷氨酰羧基酶基因，并确定了该基因的结构组成。该基因全长16.3kb，由15个外显子和14个内含子组成。DNA序列分析表明，所有14个内含子均为U2型GT-AG内含子。克隆的基因5‘侧翼区的2.8kb DNA片段在一个已知表达内源性羧基酶基因的大鼠肝细胞系中显示了转录活性。对这个5‘侧翼序列的近331bp的DNA序列分析表明，没有可识别的TATA盒。鉴定了几个转录因子的共同序列，这些转录因子可能在调节其组织特异性表达方面发挥重要作用。大鼠羧基酶基因的分离和鉴定为分析其在体外和转基因动物模型中的调控提供了必要的信息。
The vitamin K-dependent γ-glutamyl carboxylase gene was cloned from a rat liver genomic DNA library and the structural organization of this gene was determined. The carboxylase gene is 16.3 kb in length and contains 15 exons and 14 introns. DNA sequence analysis revealed that all 14 introns were U2-Type GT-AG introns. A 2.8-kb DNA fragment corresponding to the 5′-flanking region of the cloned gene demonstrated transcriptional activity in a rat liver cell line that is known to express the endogenous carboxylase gene. DNA sequence analysis of the proximal 331 bp of this 5′-flanking sequence reveals the absence of an identifiable TATA box. Consensus sequences for several transcription factors that may be important in regulating its tissue specific expression were identified. The isolation and characterization of the rat carboxylase gene provides essential information for the analysis of its regulationin vitroand in transgenic animal models.