Gene silencing in Caenorhabditis elegans by transitive RNA interference
Gene silencing in Caenorhabditis elegans by transitive RNA interference
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DOI：
10.1261/rna.2650903
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发表时间：
2003-01-01
期刊：
影响因子：
4.5
通讯作者：
Mango, SE
中科院分区：
文献类型：
--
作者：
Alder, MN;Dames, S;Mango, SE
当细胞暴露于双链RNA（dsRNA）时，来自同源基因的mRNA通过称为RNA干扰（RNAi）的过程被选择性降解。在这里，我们提供的证据表明，dsRNA在秀丽隐杆线虫中扩增，以确保强大的RNAi反应。我们的数据表明了一个模型，其中RNAi靶向的mRNA充当5'至3'合成新双链RNA（称为传递性RNAi）的模板。引人注目的是，这种效应是非自主的：靶向在一种细胞类型中表达的基因的dsRNA可以导致在不同细胞类型中表达的第二个基因的传递性RNAi介导的沉默。这些数据表明体内合成的dsRNA可以介导系统性RNAi。
When a cell is exposed to double-stranded RNA (dsRNA), mRNA from the homologous gene is selectively degraded by a process called RNA interference (RNAi). Here, we provide evidence that dsRNA is amplified in Caenorhabditis elegans to ensure a robust RNAi response. Our data suggest a model in which mRNA targeted by RNAi functions as a template for 5' to 3' synthesis of new dsRNA (termed transitive RNAi). Strikingly, the effect is nonautonomous: dsRNA targeted to a gene expressed in one cell type can lead to transitive RNAi-mediated silencing of a second gene expressed in a distinct cell type. These data suggest dsRNA synthesized in vivo can mediate systemic RNAi.