Localization of antigen on lymph node dendritic cells after exposure to the contact sensitizer fluorescein isothiocyanate. Functional and morphological studies.
Localization of antigen on lymph node dendritic cells after exposure to the contact sensitizer fluorescein isothiocyanate. Functional and morphological studies.
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DOI：
10.1084/jem.166.6.1654
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发表时间：
1987-12-01
期刊：
影响因子：
--
通讯作者：
Fryer P
中科院分区：
文献类型：
--
作者：
Macatonia SE;Knight SC;Edwards AJ;Griffiths S;Fryer P
我们研究了参与CBA小鼠对FITC接触敏感性发展的细胞。皮肤涂抹抗原后30min引流淋巴结内树突状细胞(DC)数量增加，48h达高峰，6d恢复正常，涂皮后24 h分离的DC中可见Birbeck颗粒，提示部分DC来源于皮肤朗格汉斯细胞。DC获得FITC，到8h时有两个群体，一个强荧光，另一个弱荧光。致敏后8h~3d出现强荧光细胞，在此期间DC在体外能有效地启动正常同基因T淋巴细胞的原代增殖反应。在第3~5天，引流淋巴结内淋巴细胞数量增加。在此期间，纯化的T淋巴细胞不表达可检测到的抗原水平，但浓缩的B细胞群体在暴露于抗原后的第1、2或3天短暂地表达抗原。结果表明，在抗原暴露后的3d内，DC表达抗原并刺激T细胞增殖。我们推测，在暴露于抗原后的第一个小时内，少量的FITC选择性地结合到隐蔽细胞或淋巴结DC上，不是免疫原性的，但朗格汉斯细胞获得了高水平的抗原，进入结节，并启动了免疫反应。
We have examined the cells involved in the development of contact sensitivity to FITC in CBA mice. After skin painting with antigen, the number of dendritic cells (DC) in the draining lymph nodes increased by 30 min, was maximal at 48 h, and returned to normal by 6 d. Derivation of some DC from Langerhans' cells of the skin was indicated from the presence of Birbeck granules observed in some DC isolated 24 h after skin painting. The DC acquired FITC and by 8 h there were two populations, one highly fluorescent and the other less fluorescent. The highly fluorescent cells were present between 8 h and 3 d after sensitization, and during this period the DC were potent at initiating primary proliferative responses of normal syngeneic T lymphocytes in vitro. Between days 3 and 5 the numbers of lymphocytes in the draining lymph node increased. During this period purified T lymphocytes did not express detectable levels of antigen, but enriched B cell populations expressed antigen transiently on day 1, 2, or 3 after exposure to antigen. The results showed that, during a 3-d period after exposure to antigen, DC expressed antigen and stimulated T cell proliferation. We speculate that low amounts of FITC binding selectively to veiled cells or lymph node DC in the first hours after exposure to antigen are not immunogenic but that Langerhans' cells acquire high levels of antigen, enter the nodes, and initiate immune responses.