Bod1, a novel kinetochore protein required for chromosome biorientation.
Bod1, a novel kinetochore protein required for chromosome biorientation.
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DOI：
10.1083/jcb.200704098
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发表时间：
2007-10-22
期刊：
影响因子：
--
通讯作者：
Swedlow JR
中科院分区：
文献类型：
--
作者：
Porter IM;McClelland SE;Khoudoli GA;Hunter CJ;Andersen JS;McAinsh AD;Blow JJ;Swedlow JR
We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein conserved throughout metazoans that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. Small interfering RNA depletion of Bod1 in HeLa cells produces elongated mitotic spindles with severe biorientation defects. Bod1-depleted cells form syntelic attachments that can oscillate and generate enough force to separate sister kinetochores, suggesting that microtubule–kinetochore interactions were intact. Releasing Bod1-depleted cells from a monastrol block increases the frequency of syntelic attachments and the number of cells displaying biorientation defects. Bod1 depletion does not affect the activity or localization of Aurora B but does cause mislocalization of the microtubule depolymerase mitotic centromere- associated kinesin and prevents its efficient phosphorylation by Aurora B. Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles.
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影响因子：
1.6
作者：
EARNSHAW, WC;RATRIE, H;STETTEN, G
通讯作者：
STETTEN, G
小分子黄质蛋白揭示了Aurora B在校正动型微管附着和维持纺锤体组装检查点方面的作用。
DOI：
10.1083/jcb.200208092
发表时间：
2003-04-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hauf S;Cole RW;LaTerra S;Zimmer C;Schnapp G;Walter R;Heckel A;van Meel J;Rieder CL;Peters JM
通讯作者：
Peters JM
影响因子：
12.3
作者：
Meraldi P;McAinsh AD;Rheinbay E;Sorger PK
通讯作者：
Sorger PK
影响因子：
2.9
作者：
HARDING, SE;COLFEN, H
通讯作者：
COLFEN, H
影响因子：
9.2
作者：
Lan, WJ;Zhang, X;Stukenberg, PT
通讯作者：
Stukenberg, PT