Handheld laser-induced fluorescence detection systems with different optical configurations
Handheld laser-induced fluorescence detection systems with different optical configurations
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DOI：
10.1016/j.talanta.2021.122329
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发表时间：
2021-03-31
期刊：
影响因子：
6.1
通讯作者：
Fang，Qun
中科院分区：
文献类型：
--
作者：
Peng，Ya-Mei;Pan，Jian-Zhang;Fang，Qun
小型化的激光诱导荧光（LIF）检测系统在许多研究领域中有着越来越迫切的需求。在这项工作中，小型化的LIF检测器与三种不同的光学配置的正交，共焦，和倾斜的开发，使用激光二极管作为激发源和光电二极管作为光电探测器。采用计算机模拟和实验相结合的方法研究了探测窗口附近激光散射光和荧光的分布。对溶液制备、样品流速、排列方式、过滤器模型等条件进行了优化。在优化的条件下，荧光素钠在正交和共聚焦LIF检测器上的检测限分别为40 pM和50 pM，而在倾斜LIF检测器上的检测限分别为1 nM（45°）和7 nM（67.5°）。我们进一步构建了一个完全集成的手持式正交LIF检测器，总尺寸为50 × 20 × 46 mm3，成本为380美元，对荧光素钠的检测限为10 pM。期望这种LIF检测器可以作为便携式仪器应用于现场分析或作为检测模块应用于其他分析系统中。
There is a growing urgent requirement for miniaturized laser-induced fluorescence (LIF) detection systems in many research fields. In this work, miniaturized LIF detectors with three different optical configurations of orthogonal, confocal, and oblique were developed, using a laser diode as the excitation source and a photodiode as the photodetector. The computer simulation and experimental methods were used to investigate the distributions of laser scattered light and fluorescent light near the detection window. Other conditions including the solution preparation, sample flow rate, alignment method and filter model were also optimized. Under the optimized conditions, the detection limits of sodium fluorescein for orthogonal and confocal LIF detectors were 40 pM and 50 pM, respectively, while the limit of detection (LOD) for oblique LIF detector were 1 nM (45°) and 7 nM (67.5°). We further built a fully integrated handheld orthogonal LIF detector with a total size of 50 × 20 × 46 mm3, a cost of $380, and a detection limit of 10 pM for sodium fluorescein. It is expected that such a LIF detector could be applied in field analysis as a portable instrument or in other analysis systems as a detection module.