Hypothalamic Targets for Prolactin: Assessment of c-Fos Induction in Tyrosine Hydroxylase- and Proopiomelanocortin-Containing Neurones in the Rat Arcuate Nucleus following Acute Central Prolactin Administration
Hypothalamic Targets for Prolactin: Assessment of c-Fos Induction in Tyrosine Hydroxylase- and Proopiomelanocortin-Containing Neurones in the Rat Arcuate Nucleus following Acute Central Prolactin Administration
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催乳素的下丘脑目标：急性中枢催乳素给药后大鼠弓状核中含有酪氨酸羟化酶和阿片黑皮素原的神经元中 c-Fos 诱导的评估
DOI：
10.1159/000054705
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发表时间：
2001
影响因子：
4.1
通讯作者：
D. Tortonese
中科院分区：
文献类型：
--
作者：
Belinda J. Cave;J. Wakerley;S. Luckman;D. Tortonese
催乳素（PRL）参与中枢作用，包括其自身调节和/或抑制促性腺激素分泌的作用。然而，尚不清楚哪些神经系统可能介导PRL的中枢作用。在这里，使用双重免疫组化c-Fos和酪氨酸羟化酶（TH）或proopiomelanocortin（POMC），我们已经评估了神经元激活，集中管理后，催乳素，在两个神经元网络，已被证明参与生殖功能的抑制性调节。将插管插入侧脑室后5天，雄性大鼠接受一次侧脑室注射PRL（5 µg）或生理盐水（溶剂对照）。给药后90分钟，用4%多聚甲醛灌注动物，取出大脑，在整个下丘脑区域切割30 µm冷冻切片。对平行切片进行c-Fos免疫反应性（ir）和TH-ir或POMC-ir的处理。PRL增加了弓状核吻端内c-Fos-ir神经元的平均数量（PRL和对照大鼠分别为9.3 ± 2.0 vs.5.0 ± 1.2细胞/切片; p < 0.05）。在TH阳性神经元中，PRL诱导中弓状核背内侧部c-Fos表达显著增加（p < 0.05）。与此相反，弓状核POMC神经元内c-Fos的表达没有显著增加。PRL还诱导视上核（SON）中的c-Fos表达（PRL和对照大鼠分别为11.7 ± 3.2 vs. 3.0 ± 1.4个细胞/切片; p < 0.05），但不在内侧视前核、腹内侧核或背内侧核中，这些区域据报道含有促性腺激素释放激素神经元或表达PRL受体。这项研究的结果表明，急性PRL给药后，下丘脑弓状核和SON内的早期基因立即激活。虽然PRL反应神经元在SON中的作用仍有待阐明，这些研究结果支持这样的概念，即PRL的中枢作用可以通过背内侧弓状核的TH神经元和/或在吻侧弓状核中的既不含TH也不含POMC的神经元群介导。
Prolactin (PRL) has been implicated in central actions including those that result in its own regulation and/or the suppression of gonadotropin secretion. It is not clear, however, which neuronal systems may mediate the central effects of PRL. Here, using dual immunohistochemistry for c-Fos and either tyrosine hydroxylase (TH) or proopiomelanocortin (POMC), we have assessed neuronal activation, following centrally administered PRL, within two neuronal networks that have been shown to participate in the inhibitory regulation of reproductive function. Male rats received one intracerebroventricular injection of either PRL (5 µg) or saline (vehicle control) 5 days after cannulae were inserted into the lateral ventricles. Ninety minutes after treatment, animals were perfused with 4% paraformaldehyde, the brains were removed and 30-µm frozen sections were cut throughout the entire hypothalamic region. Parallel sets of sections were processed for both c-Fos immunoreactivity (ir) and either TH-ir or POMC-ir. PRL increased the mean number of c-Fos-ir neurons within the rostral arcuate nucleus (9.3 ± 2.0 vs. 5.0 ± 1.2 cells/section, for PRL and control rats, respectively; p < 0.05). Within the TH-ir neurones, PRL induced a significant increase in c-Fos in the dorsomedial portion of the mid-arcuate nucleus (p < 0.05). In contrast, there was no significant increase in the expression of c-Fos within the POMC neurones of the arcuate nucleus. PRL also induced c-Fos expression in the supraoptic nucleus (SON) (11.7 ± 3.2 vs. 3.0 ± 1.4 cells/section for PRL and control rats, respectively; p < 0.05), but not in the medial preoptic nucleus, ventromedial nucleus or the dorsomedial nucleus, areas reported to either contain gonadotropin-releasing hormone neurones or express PRL receptors. The results from this study show immediate early gene activation within both the arcuate nucleus and the SON of the hypothalamus following acute PRL administration. While the role of PRL-responsive neurones in the SON remains to be elucidated, these findings support the notion that the central actions of PRL could be mediated via the TH neurones of the dorsomedial arcuate nucleus and/or by a population of neurones in the rostral arcuate nucleus that contain neither TH nor POMC.
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影响因子：
4.8
作者：
M. Smith
通讯作者：
M. Smith
催乳素和睾酮诱导的睾丸切除术后 LH 分泌抑制：儿茶酚胺能神经元终止于布罗卡对角带、内侧视前核和正中隆起的作用。
DOI：
10.1677/joe.0.1480291
发表时间：
1996
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Park,SK;Strouse,DA;Selmanoff,M
通讯作者：
Selmanoff,M
催乳素刺激哺乳大鼠释放催产素：通过神经叶作用发挥生理作用的证据。
影响因子：
4.1
作者：
Parker,SL;Armstrong,WE;Sladek,CD;Grosvenor,CE;Crowley,WR
通讯作者：
Crowley,WR
雄性大鼠外源性催乳素给药对去势后黄体生成激素分泌的剂量依赖性抑制：研究高催乳素性性腺功能减退症的模型。
影响因子：
4.1
作者：
Park,SK;Selmanoff,M
通讯作者：
Selmanoff,M
弓状区酪氨酸羟化酶和 POMC mRNA 因去势和高催乳素血症而增加。
DOI：
10.1016/0169-328x(91)90072-6
发表时间：
1991
期刊：
Brain research. Molecular brain research
影响因子：
--
作者：
Selmanoff,M;Shu,C;Hartman,RD;Barraclough,CA;Petersen,SL
通讯作者：
Petersen,SL