Collagenase in Human Head and Neck Tumors and Rat Tumors and Fibroblasts in Monolayer Cultures
Collagenase in Human Head and Neck Tumors and Rat Tumors and Fibroblasts in Monolayer Cultures
复制标题
人头颈肿瘤和大鼠肿瘤以及单层培养物中成纤维细胞中的胶原酶
DOI：
10.1177/000348948609500209
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Abramson
中科院分区：
文献类型：
--
作者：
Cheng;A. Blitzer;M. Abramson
侵袭性肿瘤必须释放胶原酶来分解周围的宿主结缔组织。这种酶的细胞起源尚不清楚。我们用抗胶原酶抗体定位人头颈部肿瘤和大鼠肿瘤组织中的胶原酶。胶原酶似乎定位于肿瘤结缔组织间质中，但不在肿瘤细胞中。用大鼠肿瘤细胞条件培养基处理的单层培养的大鼠皮肤成纤维细胞表现出明显的核染色和颗粒染色。未处理的成纤维细胞未显示染色。培养物中的肿瘤细胞也未显示染色。培养液的测定表明，只有加入肿瘤细胞条件培养基的成纤维细胞产生胶原酶。这些发现表明：1）胶原酶是由结缔组织基质中的成纤维细胞产生的，而不是由肿瘤细胞产生的; 2）肿瘤细胞和成纤维细胞之间的细胞相互作用似乎参与了肿瘤细胞侵袭宿主结缔组织的破坏; 3）肿瘤细胞释放可溶性因子，刺激成纤维细胞产生胶原酶。
Invasive tumors must release collagenase to break down the surrounding host connective tissues. The cellular origin of this enzyme is still unclear. We used anticollagenase antibodies to localize collagenase in the human head and neck tumor and rat tumor tissues. Collagenase appeared to be localized in the tumor connective tissue stroma but not in tumor cells. The rat skin fibroblasts in monolayer culture treated with the rat tumor cell-conditioned medium demonstrated marked extranuclear and particulate staining. Fibroblasts without treatment showed no staining. Tumor cells in the culture also showed no staining. Assay of culture media demonstrated that only fibroblasts with the addition of the tumor cell-conditioned medium produced collagenase. These findings suggest the following: 1) collagenase is produced by fibroblasts which are harbored in the connective tissue stroma, but not by tumor cells; 2) cellular interaction between tumor cells and fibroblasts appears to be involved in breakdown of the host connective tissue for tumor cell invasion; and 3) tumor cells release soluble factors which stimulate production of collagenase by fibroblasts.