Germline stem cells and follicular renewal in the postnatal mammalian ovary
Germline stem cells and follicular renewal in the postnatal mammalian ovary
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DOI：
10.1038/nature02316
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发表时间：
2004-03-11
期刊：
影响因子：
64.8
通讯作者：
Tilly, JL
中科院分区：
文献类型：
--
作者：
Johnson, J;Canning, J;Tilly, JL
生殖生物学的一个基本理论是，大多数哺乳动物雌性在胎儿期失去了生殖细胞更新的能力，因此在出生时就被赋予了封闭在卵泡内的生殖细胞（卵母细胞）的固定储备。在这里，我们表明，幼年和成年小鼠卵巢具有有丝分裂活性的生殖细胞，根据卵母细胞变性（闭锁）和清除率，需要不断补充卵泡池。与此相一致的是，用有丝分裂生殖细胞毒物白消安治疗青春期前的雌性小鼠，在成年早期消除了原始卵泡储备，而没有诱导闭锁。此外，我们证明了在幼年和成年小鼠卵巢中表达减数分裂进入标志物联会复合体蛋白3的细胞。将野生型卵巢移植到普遍表达绿色荧光蛋白（GFP）的转基因雌性小鼠中，GFP阳性生殖细胞浸润形成卵泡。总的来说，这些数据建立了生殖细胞的增殖，维持卵母细胞和卵泡生产的哺乳动物出生后的卵巢。
A basic doctrine of reproductive biology is that most mammalian females lose the capacity for germ-cell renewal during fetal life, such that a fixed reserve of germ cells (oocytes) enclosed within follicles is endowed at birth. Here we show that juvenile and adult mouse ovaries possess mitotically active germ cells that, based on rates of oocyte degeneration (atresia) and clearance, are needed to continuously replenish the follicle pool. Consistent with this, treatment of prepubertal female mice with the mitotic germ-cell toxicant busulphan eliminates the primordial follicle reserve by early adulthood without inducing atresia. Furthermore, we demonstrate cells expressing the meiotic entry marker synaptonemal complex protein 3 in juvenile and adult mouse ovaries. Wild-type ovaries grafted into transgenic female mice with ubiquitous expression of green fluorescent protein (GFP) become infiltrated with GFP-positive germ cells that form follicles. Collectively, these data establish the existence of proliferative germ cells that sustain oocyte and follicle production in the postnatal mammalian ovary.