Paper-based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity
Paper-based fluorescent sensor for rapid naked-eye detection of acetylcholinesterase activity and organophosphorus pesticides with high sensitivity and selectivity
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DOI：
10.1016/j.bios.2016.07.022
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发表时间：
2016-12-15
影响因子：
12.6
通讯作者：
Li, Feng
中科院分区：
文献类型：
--
作者：
Chang, Jiafu;Li, Haiyin;Li, Feng
已经提出了各种策略来传感乙酰胆碱酯酶（AChE）活性和有机磷农药（OPs）。然而，大多数方法的实际应用受到其固有缺点的限制，如复杂性，分析时间长，成本高。因此，开发简单、快速、灵敏的AChE活性和OPs检测方法是非常必要的。基于四苯乙烯（TPE）的聚集诱导发射（AIE）效应和马来酰亚胺的加成反应能力，我们研制了一种简单的纸基荧光传感器（PFS），该传感器已被用作快速肉眼检测AChE活性和OPs的有力工具。TPE的引入使探针在固态下具有独特的荧光性质，对提高PFS的灵敏度具有重要意义。乙酰胆碱酯酶催化硫代乙酰胆碱水解产物诱导马来酰亚胺环的破坏，激活TPE的荧光性能。考虑到AChE活性可被OP特异性抑制，所提出的PFS也可用于OP的灵敏检测。同时，荧光信号的变化可以用肉眼检测，AChE和OPs的检测限分别为2.5 mU mL（-1）和0.5 ng mL（-1）。该方法已成功应用于稀释人血清样品中AChE活性和OPs的检测，显示了其在真实的样品中应用的巨大潜力。因此，该策略具有简单、快速检测、便携性、成本效益和可视化的显著优点。(C)2016爱思唯尔B. V.保留所有权利。
Various strategies have been proposed for the sensing of acetylcholinesterase (AChE) activity and organophosphorus pesticides (OPs). However, the practical application of most methods is restricted by their intrinsic drawbacks such as complexity, long analysis time, and high cost. Thus, it is highly desirable to develop simple, fast and sensitive approaches for AChE activity and OPs detection. Herein, we reported a simple paper-based fluorescent sensor (PFS) based on the aggregation induced emission (AIE) effect of tetraphenylethylene (TPE) and the addition reaction capability of maleimide, which has been used as a powerful tool for rapid naked-eye detection of AChE activity and OPs. The introduction of TPE provides the probe with unique fluorescence property in solid state and is of great importance for improving the sensitivity of PFS. The hydrolysis product of acetylthiocholine catalyzed by AChE induced the maleimide ring destruction and activated the fluorescence performance of TPE. Given that AChE activity can be specifically inhibited by OPs, the as-proposed PFS can also be utilized for sensitive detection of OPs. Meanwhile, the variation of fluorescence signal can be readily detected by naked eyes, and low detection limits of 2.5 mU mL(-1) and 0.5 ng mL(-1) for AChE activity and OPs are obtained, respectively. Moreover, it has been successfully applied for AChE activity and OPs detection in diluted human serum samples, showing its great potential to be applied in real samples. Thus, this strategy possesses considerable advantages of simplicity, rapid detection, portability, cost efficiency and visualization. (C) 2016 Elsevier B.V. All rights reserved.