The fauna of freshwater calanoid copepods in Japan in the early decades of the 21st Century: Implications for the assessment and conservation of biodiversity
The fauna of freshwater calanoid copepods in Japan in the early decades of the 21st Century: Implications for the assessment and conservation of biodiversity
复制标题
21世纪初期日本淡水桡足类动物群：对生物多样性评估和保护的启示
DOI：
10.1002/lno.10667
复制
发表时间：
2017
影响因子：
4.5
通讯作者：
Urabe Jotaro
中科院分区：
文献类型：
--
作者：
Makino Wataru;Tanabe Akifumi S.;Urabe Jotaro
在桡足类动物中，经常发现形态上无法区分但基因上不同的神秘物种复合体，例如日本湖泊和池塘中常见的太平洋棘皮鱼复合体。在本研究中，利用分子技术对日本淡水桡足类桡足类动物群进行了广泛的重新检查，并根据分子鉴定推导了每种桡足类物种的自生态学。在日本各地收集样本，通过其形态诊断特征进行鉴定，然后对线粒体细胞色素氧化酶亚基 1 (mtCOI) 进行测序。本研究总共发现了 13 个物种，并且在大多数情况下，每个水体仅采集了 1 个物种。 TheA. pacificuscomplex不仅出现在高山湖泊和山地沼泽的水池中，而且还出现在低海拔平原的水体中。其他物种的出现仅限于低海拔水体，且多为人工灌溉池塘。日本特有的Heliodiaptomus nipponicus和Neutrodiaptomus formosus仅在灌溉池塘中发现，表明目前人工水体对于维持日本淡水浮游动物的多样性是不可或缺的。 mtCOI 测序结果揭示了假定的神秘物种复合体的存在，例如日本的 Neutrodiaptomus formosusus，以及亚洲范围内的 Neodiaptomus schmackeri、Sinodiaptomus sarsi、Heliodiaptomus kikuchii 和 Eurytemora affinisa。因此，强烈建议淡水浮游动物监测计划的任何结果都应补充 DNA 序列，以便使该结果对于未来的研究来说也是合理可靠的。
In copepods, morphologically‐indistinguishable but genetically‐different cryptic species complexes are frequently found, such as theAcanthodiaptomus pacificuscomplex that is commonly found in Japanese lakes and ponds. In the present study, the fauna of Japanese freshwater calanoid copepods were extensively reexamined using molecular techniques, and the autecology of each calanoid species was deduced based on the molecular identifications. Specimens were collected throughout Japan, identified by their morphological diagnostic characteristics, and then sequenced for the mitochondrial cytochromecoxidase subunit 1 (mtCOI). In total, 13 species were found in the present study, and in most cases, only one species was collected from each waterbody. TheA. pacificuscomplex occurred not only in high mountain lakes and pools of mountain moors but also in water bodies on low‐altitude plains. The occurrence of the other species was restricted to low‐altitude waterbodies, which were mostly man‐made irrigation ponds.Heliodiaptomus nipponicusandNeutrodiaptomus formosus, which are endemic to Japan, were found only in irrigation ponds, indicating that artificial waterbodies are currently indispensable to maintain the diversity of Japanese freshwater zooplankton. The results of mtCOI sequencing revealed the existence of putative cryptic species complexes such asNeutrodiaptomus formosusin Japan, andNeodiaptomus schmackeri,Sinodiaptomus sarsi,Heliodiaptomus kikuchii, andEurytemora affinisacross their Asian range. It is thus strongly recommended that any outcome of the freshwater zooplankton monitoring programs always be supplemented with DNA sequences in order to render that outcome sound and reliable also for future studies.