Activin and transforming growth factor-β as local regulators of ovarian steroidogenesis in the goldfish
Activin and transforming growth factor-β as local regulators of ovarian steroidogenesis in the goldfish
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DOI：
10.1016/s0016-6480(03)00060-1
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发表时间：
2003-06-01
影响因子：
2.7
通讯作者：
Van Der Kraak, G
中科院分区：
文献类型：
--
作者：
Calp, MK;Matsumoto, JA;Van Der Kraak, G
本研究探讨了激活素和 TGFbeta(1) 作为金鱼卵巢功能局部调节因子的假设。初步研究通过使用 RT-PCR 证明了卵巢中存在 TGFbeta，该 RT-PCR 扩增了来自早期卵黄发生 (EVIT) 和早熟完全生长 (PFG) 卵泡的 225 bp 产物。该转录本与其他硬骨鱼中的 TGFbeta 具有高度同源性。金鱼重组激活素 B 和人重组 TGFbeta(1) 均抑制体外培养的 EVIT 卵泡产生基础睾酮。激活素 B 还可以抑制 EVIT 卵泡由 hCG 刺激的睾酮产生。我们的实验表明，激活素 B 通过在类固醇生成途径中胆固醇形成和/或动员上游位点的作用以及通过独立于 cAMP 形成作用的机制来介导这些作用。在 PFG 卵泡实验中，TGFbeta(1) 降低了基础睾酮的产生。激活素 B 不影响 PFG 卵泡产生 T，这表明该激素对金鱼卵巢中类固醇生成的影响不同，具体取决于卵巢成熟的阶段。在其他 PFG 卵泡测试中，TGFbeta(1) 和激活素 B 在一定程度上抑制 17α-OHP 转化为成熟诱导激素 17α,20β-二羟基-4-孕烯-3-酮。总之，这项研究表明 TGF 在金鱼卵巢中表达，并且激活素和 TGFbeta 都会影响类固醇的产生，这提供了证据表明 TGFbeta 超家族的这些成员可能充当硬骨鱼卵巢功能的局部调节剂。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
This study explores the hypothesis that activin and TGFbeta(1) serve as local regulators of ovarian function, in the goldfish. Initial studies demonstrated the presence of TGFbeta in the ovary through the use RT-PCR, which amplified a 225 bp product from early vitellogenic (EVIT) and prematurational full-grown (PFG) follicles. This transcript showed high homology to TGFbeta in other teleosts. Both goldfish recombinant activin B and human recombinant TGFbeta(1) suppressed basal testosterone production by EVIT follicles incubated in vitro. Activin B also inhibited hCG-stimulated testosterone production by EVIT follicles. Our experiments suggest that activin B mediates these effects through actions at sites upstream of cholesterol formation and/or mobilization in the steroidogenic pathway, and through mechanisms that were independent of effects on cAMP formation. In experiments with PFG follicles, TGFbeta(1) decreased basal testosterone production. Activin B did not affect T production by PFG follicles, suggesting that this hormone has differential effects on steroidogenesis in the goldfish ovary depending on the stage of ovarian maturity. In other tests with PFG follicles, TGFbeta(1) and activin B, to a limited extent, inhibited the conversion of 17alpha-OHP to the maturation-inducing hormone, 17alpha,20beta-dihydroxy-4-pregnen-3-one. In conclusion, this study shows that TGF is expressed in the goldfish ovary, and that both activin and TGFbeta affect steroid production, which provides evidence that these members of the TGFbeta superfamily may act as local regulators of ovarian function in a teleost. (C) 2003 Elsevier Science (USA). All rights reserved.