Scanning electron microscope and micro-CT evaluation of cranial sutures in health and disease
Scanning electron microscope and micro-CT evaluation of cranial sutures in health and disease
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DOI：
10.1097/01.scs.0000230019.46896.b0
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发表时间：
2006-09-01
影响因子：
0.9
通讯作者：
Self, Peter
中科院分区：
文献类型：
--
作者：
Anderson, Peter J.;Netherway, David J.;Self, Peter
通过对正常颅缝（很少发生颅缝早闭）的形态学研究，已经确定了目前关于颅缝生物学的知识。这些最初通常使用组织学研究，或最近使用CT扫描作为研究工具，但通常使用动物模型。然而，最近的技术进步提供了潜力，以完善我们的理解超微结构的使用新的先进的扫描技术，这提供了更详细的分辨率的可能性。我们的目标是进行详细的扫描正常，融合和融合的缝线从患者颅缝影响不同的缝线，使用现代显微CT扫描仪和显微分析扫描电子显微镜研究融合过程不同阶段的详细结构。我们希望在我们的研究中包括所有的人类缝线，因为以前的研究大多是使用矢状缝进行的。来自7名患者的10根缝线揭示了复杂的超微结构排列。在融合的矢状缝的颅外表面和颅内表面上观察到的不同的骨隆起模式在融合的冠状缝或齿状缝的更近检查中没有重复。元素分析证实，钙的量增加，碳的量减少，采样区域远离suture margin.We的结论，扫描允许详细的评估，并揭示了复杂的安排的结构的人颅缝和那些正在进行的过程中的颅缝早闭，最终结构取决于受影响的缝的一些差异。
Current knowledge of suture biology has been ascertained as a result of morphological studies of normal cranial sutures (and rarely those undergoing craniosynostosis). These were initially undertaken often using histological investigations, or more recently using CT scans, as investigative tools, but have often used animal models. However, recent technological advances have provided the potential to refine our understanding of the ultrastructure by the use of new advanced scanning technology, which offers the possibility of more detailed resolution.Our aim was to undertake detailed scans of normal, fusing and fused sutures from patients with craniosynosotosis affecting different sutures, to study the detailed structure at different stages of the fusion process using a modern micro-CT scanner and a microanalytical scanning electron microscope. We wished to include in our study all the human sutures because previous studies have mostly been undertaken using the sagittal suture.Ten sutures from seven patients have revealed a complex ultra-structural arrangement. The different patterns of bone ridging seen on the ectocranial and endocranial surfaces of the fused sagittal suture were not repeated on closer inspection of either fused coronal or lambdoid sutures. Elemental analysis confirmed that the amount of calcium increased and the amount of carbon decreased as sampled areas moved away from the suture margin.We conclude that scanning allowed detailed assessment and revealed the complex arrangement of the structure of the human cranial sutures and those undergoing the process of craniosynostosis, with some differences in final structure depending on the affected suture.