Beta-galactosidase in the seminal plasma and reproductive organs of the bull.
Beta-galactosidase in the seminal plasma and reproductive organs of the bull.
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公牛精浆和生殖器官中的β-半乳糖苷酶。
DOI：
10.1016/0020-711x(86)90395-2
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
T. Vanha
中科院分区：
文献类型：
--
作者：
A. Jauhiainen;T. Vanha
研究了公牛不同生殖器官、精浆和精子中β-半乳糖苷酶活性的分布，发现在睾丸和附睾的不同部位β-半乳糖苷酶的比活性最高，其活性似乎部分以分泌形式（尾部分泌），部分以非分泌形式（附睾头至尾部）。在pH 7.0的Sepharose 6 B上进行凝胶过滤，显示生殖器官和精浆中有两种β-半乳糖苷酶形式（GF-1，Mr约500，000 - 600，000和GF-2，Mr约190，000 - 220，000）。两种β-半乳糖苷酶形式的最适pH为约3.75-4.75。Hg ~（2+）和对氯汞苯甲酸盐强烈抑制这些活性。此外，GF-2型似乎比GF-1型对50-70 ℃下的热灭活稍微更敏感。在层析聚焦中，公牛精浆中的β-半乳糖苷酶活性与附睾尾的β-半乳糖苷酶活性共洗脱（pI值7.5-6.4）。相反，前列腺、考珀腺、睾丸、壶腹和精囊在较低的pI值（6.3-4.2）下具有酶活性洗脱。因此，精浆活性主要是附睾尾功能的指标。
The distribution of beta-galactosidase activity was studied in different reproductive organs, seminal plasma and spermatozoa of the bull. The highest specific activity of beta-galactosidase was found in testis and in different parts of the epididymis, where the activity seemed to be partly in secretory (cauda secretion) and partly in non-secretory, bound form (caput to cauda epididymidis). Gel filtration on Sepharose 6B at pH 7.0 revealed two beta-galactosidase forms (GF-1, Mr approximately 500,000-600,000 and GF-2, Mr approximately 190,000-220,000) in reproductive organs and seminal plasma. The pH-optimum of both beta-galactosidase forms was about 3.75-4.75. Hg2+ and p-chloromercuribenzoate inhibited strongly these activities. Further, form GF-2 seemed to be slightly more sensitive to the thermal inactivation at 50-70 degrees C than form GF-1. In chromatofocusing beta-galactosidase activities in bull seminal plasma coeluted with those of the cauda epididymidis (pI-values 7.5-6.4). On the contrary, prostate, Cowper's gland, testis, ampulla and seminal vesicles had enzyme activities eluting at lower pI-values (6.3-4.2). Thus, the seminal plasma activity is mainly an indicator for the function of the epididymal cauda.