Epitope analysis of birch pollen allergen in Japanese subjects
Epitope analysis of birch pollen allergen in Japanese subjects
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DOI：
10.1023/a:1027323811296
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发表时间：
1997-11-01
影响因子：
9.1
通讯作者：
Katagiri, M
中科院分区：
文献类型：
--
作者：
Abe, Y;Kimura, S;Katagiri, M
桦树花粉不仅在斯堪的纳维亚半岛、欧洲、加拿大和美国的北方地区，而且在日本的北海道，都是鼻过敏（花粉症）的一个非常常见的原因。我们以前曾报道过日本人桦树花粉过敏症的发生与HLA-DR 9表型呈正相关。然而，很少有关于桦树花粉的T细胞表位的信息，这些表位是由HLA-DR 9以外的HLA II类分子呈递的。因此，我们分析了具有HLA-DR 9的患者与没有HLA-DR 9的患者在T细胞表位使用方面的差异。研究了7例日本桦树花粉症患者。一些代表T细胞表位的肽组（疣皮桦; Bet VI肽，p7-33，p23-46，p138-160）似乎被大多数人共享，而另一种肽（Bet VI p72-95）主要被表达HLA-DR 9和/或HLA-DQ 3分子的患者识别。此外，从两名不具有HLA-DR 9或HLA-DQ 3的患者产生了七个T细胞克隆和八个T细胞系。使用这些T细胞克隆/系中的一些，我们研究了HLA II类分子和抗原肽之间的关系。这些T细胞克隆中的一个在HLA-DQ 1分子的背景下识别抗原肽。据我们所知，这是Bet VI上的表位可以由HLA-DQ分子呈递的第一个迹象。
Birch pollen is a very common cause of nasal allergy (pollinosis) not only in Scandinavia, Europe, Canada, and the northern part of the United States but also in Hokkaido, Japan. We have previously reported a positive association between the HLA-DR9 phenotype and the development of birch pollen allergy in Japanese subjects. However, there is little information about T cell epitopes of birch pollen which are presented by HLA class II molecules other than HLA-DR9. Therefore, we analyzed the difference in T cell epitope usage in patients who had HLA-DR9 versus those who did not. Seven Japanese patients with birch pollinosis were studied. Some groups of peptides representing T cell epitopes (Betula verrucosa; Bet VI peptides, p7-33, p23-46, p138-160) appeared to be shared by the majority, while another peptide (Bet VI p72-95) was recognized predominantly by patients who expressed HLA-DR9 and/or HLA-DQ3 molecules. Moreover, seven T cell clones and eight T cell lines were generated from two patients who did not have HLA-DR9 or HLA-DQ3. Using some of these T cell clones/lines, we investigated the relationship between HLA class II molecules and antigenic peptides. One of these T cell clones recognized antigenic peptides in the context of the HLA-DQ1 molecule. To our knowledge, this is the first indication that the epitope on Bet VI can be presented by the HLA-DQ molecule.