Endophytic Streptomyces sp Y3111 from traditional Chinese medicine produced antitubercular pluramycins
Endophytic Streptomyces sp Y3111 from traditional Chinese medicine produced antitubercular pluramycins
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DOI：
10.1007/s00253-013-5335-6
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发表时间：
2014-02-01
影响因子：
5
通讯作者：
Zhang, Lixin
中科院分区：
文献类型：
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作者：
Liu, Miaomiao;Abdel-Mageed, Wael M.;Zhang, Lixin
作为从天然来源寻找抗结核物质的一部分，我们筛选了一个从中药中分离的内生微生物文库（共50株和300个粗提物），以测定其对卡介苗（BCG）的生长抑制活性。菌株Y3111粗提物抗卡介苗活性较好，MIC值为12.5 μ g/mL。生物测定引导分离得到4个新的多霉素类化合物heraclemycins A-D（1-4）。通过不同的光谱技术，包括HRMSESI， 1D NMR和2D NMR来确定它们的结构。这是中医药内生微生物产生的多霉素类似物的第一篇报道，也是首个bcg选择性多霉素的例子。Heraclemycin C(3)对BCG具有选择性抗结核活性，MIC值为6.25 μ g/mL，可能是一种新的作用方式。
As part of a search for antitubercular substances from natural sources, we screened a library of endophytic microbes (50 strains and 300 crude extracts in total) isolated from traditional Chinese medicines (TCMs) for growth inhibitory activity against Bacillus Calmette-Gu,rin (BCG). The crude extract of Streptomyces sp. strain Y3111, which was associated with the stems of Heracleum souliei, showed good anti-BCG activity with an MIC value of 12.5 mu g/mL. Bioassay-guided isolation led to four new pluramycin-type compounds, heraclemycins A-D (1-4). Their structures were determined by different spectroscopic techniques including HRMSESI, 1D NMR, and 2D NMR. This is the first report of pluramycin analogues produced by TCM endophytic microbes as well as the first example of BCG-selective pluramycins. Heraclemycin C (3) showed selective antitubercular activity against BCG with a MIC value of 6.25 mu g/mL and a potential new mode of action.