Losses of chromosomal arms 1p and 19q in the diagnosis of oligodendroglioma. A study of paraffin-embedded sections
Losses of chromosomal arms 1p and 19q in the diagnosis of oligodendroglioma. A study of paraffin-embedded sections
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DOI：
10.1038/modpathol.3880400
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发表时间：
2001-09-01
期刊：
影响因子：
7.5
通讯作者：
Feuerstein, BG
中科院分区：
文献类型：
--
作者：
Burger, PC;Minn, AY;Feuerstein, BG
比较基因组杂交（CGH），荧光原位杂交（FISH），聚合酶链反应为基础的微卫星分析，和p53测序进行石蜡包埋材料从18少突胶质细胞瘤和组织学相似的星形细胞瘤。进行这项研究是因为有证据表明，1p和19q染色体臂的同时丢失是少突胶质细胞瘤的特异性标志。在6个回顾性诊断为少突胶质细胞瘤的病变中，通过CGH、FISH和聚合酶链反应观察到所有病变均存在1p和19q的预测丢失。其他病变，包括一些考虑少突胶质细胞瘤。或混合型神经胶质瘤，但没有。在6例少突胶质细胞瘤中没有发现p53突变，而其余10例成功研究的病例中有5例确实存在p53突变。结果表明，对当前或存档组织进行的CGH和FISH可以帮助浸润性胶质瘤（如少突胶质细胞瘤和星形细胞瘤）的分类。p53研究的结果与以前的研究结果一致，即这种突变在少突胶质细胞瘤中比在星形细胞瘤中更不常见。
Comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), polymerase chain reaction-based microsatellite analysis, and p53 sequencing were performed in paraffin-embedded material from 18 oligodendrogliomas and histologically similar astrocytomas. The study was undertaken because of evidence that concurrent loss of both the 1p and 19q chromosome arms is a specific marker for oligodendrogliomas. Of the six lesions with a review diagnosis of oligodendroglioma, all had the predicted loss of 1p and 19q seen by CGH, FISH, and polymerase chain reaction. Other lesions, including some considered oligodendroglioma. or mixed glioma by the submitting institution, did not. There were no p53 mutations in any of the six oligodendrogliomas, whereas 5 of the 10 remaining, successfully studied cases did have p53 mutations. The results suggest that CGH and FISH performed on current or archival tissue can aid in classification of infiltrating gliomas such as oligodendrogliomas and astrocytomas. The results of the p53 studies are consistent with findings of previous investigations that such mutations are less common in oligodendrogliomas than they are in astrocytomas.