Tuning the erosion rate of artificial protein hydrogels through control of network topology
Tuning the erosion rate of artificial protein hydrogels through control of network topology
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DOI：
10.1038/nmat1573
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发表时间：
2006-02-01
期刊：
影响因子：
41.2
通讯作者：
Tirrell, DA
中科院分区：
文献类型：
--
作者：
Shen, W;Zhang, KC;Tirrell, DA
侵蚀行为支配物理水凝胶在生物医学应用中的使用，范围从控制释放到细胞包封。基因工程蛋白质水凝胶通过设计其氨基酸序列和网络拓扑结构提供了控制侵蚀速率的独特手段。在这里，我们表明，这种材料的侵蚀率可以通过利用选择性分子识别，离散聚集数和卷曲螺旋蛋白质结构域的方向歧视来调整。由具有不同螺旋卷曲螺旋末端结构域（P和A）的三嵌段人工蛋白形成的水凝胶比由具有相同末端结构域（P或A）的那些形成的水凝胶腐蚀慢一百倍以上。腐蚀速率的降低是环链被抑制的结果，因为P和A倾向于彼此不缔合。因此，在人工蛋白质水凝胶中，侵蚀速率可以在几个数量级上进行调整，为各种生物医学应用打开了大门。
Erosion behaviour governs the use of physical hydrogels in biomedical applications ranging from controlled release to cell encapsulation. Genetically engineered protein hydrogels offer unique means of controlling the erosion rate by engineering their amino acid sequences and network topology. Here, we show that the erosion rate of such materials can be tuned by harnessing selective molecular recognition, discrete aggregation number and orientational discrimination of coiled-coil protein domains. Hydrogels formed from a triblock artificial protein bearing dissimilar helical coiled-coil end domains ( P and A) erode more than one hundredfold slower than hydrogels formed from those bearing the same end domains ( either P or A). The reduced erosion rate is a consequence of the fact that looped chains are suppressed because P and A tend not to associate with each other. Thus, the erosion rate can be tuned over several orders of magnitude in artificial protein hydrogels, opening the door to diverse biomedical applications.