Identification of neuroblastoma stem cells by characterization of side population cells in the human neuroblastoma SK-N-SH cell line
Identification of neuroblastoma stem cells by characterization of side population cells in the human neuroblastoma SK-N-SH cell line
复制标题
通过人神经母细胞瘤 SK-N-SH 细胞系中侧群细胞的表征来鉴定神经母细胞瘤干细胞
DOI：
10.1016/j.jpedsurg.2010.08.022
复制
发表时间：
2010-12-01
影响因子：
2.4
通讯作者：
Xiao, Xianmin
中科院分区：
文献类型：
--
作者：
Qi, Shiqin;Zheng, Jicui;Xiao, Xianmin
目的：本研究从神经母细胞瘤SK-N-SH细胞系中分离出侧群（side population，SP）细胞，并对其是否具有干细胞特征进行系统研究。结果：SK-N-SH SP细胞表达高水平的干细胞标志物，具有较高的增殖能力和恶性能力，并具有快速分化的能力。非SP细胞高水平表达分化标志蛋白，增殖能力低，恶性程度低，分化缓慢。结论：SK-N-SH SP细胞具有肿瘤干细胞样特性。(C)2010年爱思唯尔公司All rights reserved.
Purpose: The purpose of the study was to sort side population (SP) cells from the neuroblastoma SK-N-SH cell line and to systematically investigate whether this population has stem cell characteristics.Methods: Side population and non-SP cells were separated from the SK-N-SH cell line by flow cytometry, and their morphologic characteristics were analyzed by light and electron microscopy. We also used Western blotting to analyze marker proteins, Cell Counting Kit-8 assay for proliferative ability, series differentiation studies for differentiation properties, and Matrigel invasion study and tumorigenicity assay for malignant potential.Results: The SK-N-SH SP cells expressed high levels of stem cell markers, had high proliferative and malignant abilities, and had the capacity for rapid differentiation. The non-SP cells expressed differentiated cell marker proteins at high levels, had low proliferative and malignant abilities, and exhibited slow differentiation.Conclusions: The SK-N-SH SP cells have cancer stem cell-like properties. (C) 2010 Elsevier Inc. All rights reserved.