Bioavailability modification and fungal biodegradation of PAHs in aged industrial soils
Bioavailability modification and fungal biodegradation of PAHs in aged industrial soils
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DOI：
10.1016/j.ibiod.2007.02.004
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发表时间：
2007
影响因子：
4.8
通讯作者：
Vanessa Leonardi;V. Šašek;M. Petruccioli;A. D’Annibale;P. Erbanová;T. Cajthaml
中科院分区：
文献类型：
--
作者：
Vanessa Leonardi;V. Šašek;M. Petruccioli;A. D’Annibale;P. Erbanová;T. Cajthaml
污染土壤中污染物的生物有效性是影响生物修复过程的重要因素。我们调查了多环芳烃（PAHs）的生物利用度对真菌修复老化基质的效果的影响。两个历史上污染的土壤，不同的两个总的PAH浓度（2320与612 mgPAHskg-1）和非生物可利用部分的相对量（4.0与32%，分别），孵育两个白色腐烂真菌-Irpex lacteus和Pleoporostreatus。当生物降解的结果与PAH生物可利用的馏分的数量估计连续超临界流体萃取，相关系数范围为0.60至0.83。为了评估真菌修复效率的可能增加，四种不同的动员剂（MAs），即大豆油，吐温20，吐温80和橄榄厂废水，被添加到土壤真菌培养之前。在土壤中添加微量的非生物可利用部分的MA对PAH降解的影响有限，甚至是负面的。另一种土壤的情况也是如此，其特点是非生物可利用部分较大。两种白色腐菌对历史污染土壤中多环芳烃的降解作用在多环芳烃含量和生物有效性上存在显著差异。然而，这两种真菌在污染物生物有效性较高的土壤中表现更好。因此，非生物可利用部分的初步测定可能会提供一个有用的指示生物修复处理的性能。
Bioavailability of pollutants in contaminated soils represents an important factor influencing the bioremediation process. We investigated the impact of the bioavailability of polycyclic aromatic hydrocarbons (PAHs) on the efficacy of mycoremediation of aged matrices. Two historically contaminated soils, differing in both overall PAH concentrations (2320 vs. 612mgPAHskg-1) and in the relative amount of the non-bioavailable fraction (4.0 vs. 32%, respectively), were incubated with two white rot fungi –Irpex lacteus and Pleurotus ostreatus. When relating biodegradation results with the amount of PAH bioavailable fractions estimated by sequential supercritical fluid extractions, the correlation coefficients ranged from 0.60 to 0.83. In order to assess a possible increase of the mycoremediation efficiency, four different mobilizing agents (MAs), i.e. soybean oil, Tween 20, Tween 80 and olive-mill wastewater, were added to soil prior to fungal incubations. The addition of MAs to the soil with a negligible amount of the non-bioavailable fraction had either a limited or even a negative impact on PAH degradation. The same was true also in the case of the other soil, characterized by a larger non-bioavailable fraction. Both white rot fungi under study markedly degraded PAHs in historically contaminated soils differing significantly in PAH content and the bioavailability. However, both the fungi performed better in the soil with higher pollutant bioavailability. Consequently, a preliminary determination of non-bioavailable fraction might provide a useful indication of the performance of bioremediation treatment.