Expression of the human chondrocyte phenotype in vitro.
Expression of the human chondrocyte phenotype in vitro.
复制标题
人软骨细胞表型的体外表达。
DOI：
10.1007/bf02623638
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Horton,WA
中科院分区：
文献类型：
--
作者：
Aulthouse,AL;Beck,M;Griffey,E;Sanford,J;Arden,K;Machado,MA;Horton,WA
我们报告了一个培养方案，其中人骺软骨细胞失去其分化的表型在单层，随后重新表达的表型在琼脂糖凝胶。该方案是基于使用兔软骨细胞的方法。单层培养允许少量细胞扩增，并为研究分化的人软骨细胞表型的表达提供了起点。单层培养的细胞产生I型前胶原、纤连蛋白和小的非软骨蛋白多糖。随后在琼脂糖中培养与典型软骨细胞超微结构特征的获得和II型胶原和软骨特异性蛋白聚糖的合成相关。在琼脂糖培养1 - 2周的条件下，从非软骨细胞到分化软骨细胞表型的转变发生在这些条件下，并且在整个培养过程中不一定是均匀的。这种培养技术将有助于直接研究人类软骨疾病，这些疾病在过去已经通过替代方法得到了解决。
We report a culture scheme in which human epiphyseal chondrocytes lose their differentiated phenotype in monolayer and subsequently reexpress the phenotype in an agarose gel. The scheme is based on a method using rabbit chondrocytes. Culture in monolayer allowed small quantities of cells to be amplified and provided a starting point to study expression of the differentiated human chondrocyte phenotype. The cells cultured in monolayer produced type I procollagen, fibronectin, and small noncartilaginous proteoglycans. Subsequent culture in agarose was associated with the acquisition of typical chondrocyte ultrastructural features and the synthesis of type II collagen and cartilage-specific proteoglycans. The switch from the nonchondrocyte to the differented chondrocyte phenotype occurred under these conditions between 1 and 2 wk of agarose culture and was not necessarily homogeneous throughout a culture. This culture technique will facilitate direct investigation of human disorders of cartilage that have been addressed in the past by alternative approaches.