Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
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DOI：
10.1371/journal.pone.0083146
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tomita K
中科院分区：
文献类型：
--
作者：
Nonoguchi H;Izumi Y;Nakayama Y;Matsuzaki T;Yasuoka Y;Inoue T;Inoue H;Mouri T;Kawahara K;Saito H;Tomita K
已知心房利钠肽（ANP）影响延髓粗升支（MAL）中的NaCl转运，其中最大的NaCl重吸收发生在响应精氨酸加压素（AVP）的远端肾单位节段之间。在本研究中，我们使用离体小管灌注技术研究了ANP对MAL中碳酸氢盐（HCO 3 −）转运的影响。HCO 3 −浓度使用自由流动超微量荧光计测量。我们首先在长环和短环MAL（分别为lMAL和sMAL）中观察到基础HCO 3 −重吸收。AVP抑制lMAL和sMAL的HCO 3 −重吸收，而ANP不改变HCO 3 −转运。然而，在AVP存在的情况下，ANP恢复了lMAL和sMAL中AVP抑制的HCO 3-重吸收。ANP对HCO 3 −转运的影响由环GMP模拟。IMALs中加压素V2受体的mRNA表达水平显著高于SMALs，而V1 a受体的表达无变化。总之，AVP抑制HCO 3 −转运，ANP抵消AVP对lMAL和sMAL中HCO 3 −转运的作用。
Atrial natriuretic peptide (ANP) is known to influence NaCl transport in the medullary thick ascending limbs (MAL), where the largest NaCl reabsorption occurs among distal nephron segments in response to arginine vasopressin (AVP). In the present study, we investigated the effect of ANP on bicarbonate (HCO3 −) transport in the MAL using an isolated tubule perfusion technique. The HCO3 − concentration was measured using free-flow ultramicro-fluorometer. We first observed basal HCO3 − reabsorption in both long- and short-looped MALs (lMALs, and sMALs, respectively). AVP inhibited HCO3 − reabsorption in both lMALs and sMALs, whereas ANP did not change HCO3 − transport. However, in the presence of AVP, ANP restored the HCO3 − reabsorption inhibited by AVP both in lMAL and sMAL. The effects of ANP on HCO3 − transport was mimicked by cyclic GMP. The mRNA expression level of the vasopressin V2 receptor in lMALs was significantly higher than in sMALs, whereas expression of the V1a receptor was unchanged. In summary, AVP inhibits HCO3 − transport, and ANP counteracts the action of AVP on HCO3 − transport both in lMALs and sMALs.
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影响因子：
13.6
作者：
Izumi, Yuichiro;Hori, Kahori;Nonoguchi, Hiroshi
通讯作者：
Nonoguchi, Hiroshi
影响因子：
15.9
作者：
CAPASSO, G;UNWIN, R;GIEBISCH, G
通讯作者：
GIEBISCH, G
DOI：
10.1152/ajprenal.00049.2006
发表时间：
2007-01-01
影响因子：
4.2
作者：
Carmosino, Monica;Brooks, Heddwen L.;Breyer, Matthew D.
通讯作者：
Breyer, Matthew D.
DOI：
10.1152/ajprenal.00176.2004
发表时间：
2004-12-01
影响因子：
4.2
作者：
Good, DW;Watts, BA;Shull, GE
通讯作者：
Shull, GE
DOI：
10.1152/ajprenal.00052.2012
发表时间：
2012-10-01
影响因子：
4.2
作者：
Hori, Kahori;Nagai, Takanori;Nonoguchi, Hiroshi
通讯作者：
Nonoguchi, Hiroshi