De novo Generation of an Axially Vascularized Processed Bovine Cancellous-Bone Substitute in the Sheep Arteriovenous-Loop Model
De novo Generation of an Axially Vascularized Processed Bovine Cancellous-Bone Substitute in the Sheep Arteriovenous-Loop Model
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DOI：
10.1159/000324408
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Kneser, U.
中科院分区：
文献类型：
--
作者：
Beier, J. P.;Hess, A.;Kneser, U.
背景/目的：本研究的目的是生成轴向血管化骨替代物。在大型动物模型中应用动静脉（AV）环方法，以在临床批准的具有主要机械稳定性的显著体积的加工牛松质骨（PBCB）基质中诱导轴向血管化，并评估增加轴向血管化的过程。方法：将PBCB结构植入13只美利奴绵羊体内，并在隔离室中植入显微手术形成的AV环。通过连续磁共振成像（MRI）扫描监测血管化过程。对外植体进行微型计算机断层扫描（micro-CT）分析、组织形态测量和CD 31和CD 45的免疫组织化学。结果：通过组织形态计量学和显微CT扫描对PBCB结构中增加的轴向血管化进行定量和可视化。活体连续MRI扫描显示基质内灌注体积显著进行性增加。免疫组化证实了新生血管的内皮衬里。结论：本研究证明了在大型动物模型中通过显微外科AV袢成功实现了临床批准的机械稳定骨替代物的轴向血管化。因此，显微外科移植的组织工程，轴向血管化和机械稳定的骨替代品与临床相关的尺寸可能成为临床可行的未来。版权所有（C）2011 S. Karger AG，巴塞尔
Background/Aims: The aim of this study was to generate an axially vascularized bone substitute. The arteriovenous (AV)-loop approach in a large-animal model was applied in order to induce axial vascularization in a clinically approved processed bovine cancellous bone (PBCB) matrix of significant volume with primary mechanical stability and to assess the course of increasing axial vascularization. Methods: PBCB constructs were implanted into 13 merino sheep together with a microsurgically created AV loop in an isolation chamber. The vascularization process was monitored by sequential magnetic resonance imaging (MRI) scans. Explants were subjected to micro-computed tomography (micro-CT) analysis, histomorphometry and immunohistochemistry for CD31 and CD45. Results: Increasing axial vascularization in PBCB constructs was quantified by histomorphometry and visualized by micro-CT scans. Intravital sequential MRI scans demonstrated a significant progressive increase in perfused volume within the matrices. Immunohistochemistry confirmed endothelial lining of newly formed vessels. Conclusion: This study demonstrates successful axial vascularization of a clinically approved, mechanically stable bone substitute with a significant volume by a microsurgical AV loop in a large-animal model. Thus microsurgical transplantation of a tissue-engineered, axially vascularized and mechanically stable bone substitute with clinically relevant dimensions may become clinically feasible in the future. Copyright (C) 2011 S. Karger AG, Basel