Halloysite Clay Nanotubes for Enzyme Immobilization.
Halloysite Clay Nanotubes for Enzyme Immobilization.
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DOI：
10.1021/acs.biomac.5b01542
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发表时间：
2016-01
影响因子：
6.2
通讯作者：
J. Tully;R. Yendluri;Y. Lvov
中科院分区：
文献类型：
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作者：
J. Tully;R. Yendluri;Y. Lvov
埃洛石粘土是通过轧制高岭石粘土的平板形成的铝硅酸盐纳米管。它们具有15 nm的管腔，50-70 nm的外径，0.5-1 μm的长度，以及不同的内部/外部化学。由于这些纳米级的特性，它们被用于装载，储存和控制释放活性化学试剂，包括防腐剂，杀菌剂和药物。研究了漆酶、葡萄糖氧化酶和脂肪酶在埃洛石上的固定化。总的来说，带负电的蛋白质在其等电点以上大部分被装载到带正电的管的内腔中。具有蛋白质的典型管负载为6-7重量%，其中三分之一在5-10小时内释放，其余三分之二保留，从而在纳米限制条件下提供增强的生物催化。固定化脂肪酶表现出增强的稳定性在酸性pH值，和最佳pH值转移到更碱性pH值。固定化漆酶是更稳定的相对于时间，和固定化葡萄糖氧化酶表现出保留酶活性高达70 °C，而本机样品是无活性的。
Halloysite clay is an aluminosilicate nanotube formed by rolling flat sheets of kaolinite clay. They have a 15 nm lumen, 50-70 nm external diameter, length of 0.5-1 μm, and different inside/outside chemistry. Due to these nanoscale properties, they are used for loading, storage, and controlled release of active chemical agents, including anticorrosions, biocides, and drugs. We studied the immobilization in halloysite of laccase, glucose oxidase, and lipase. Overall, negatively charged proteins taken above their isoelectric points were mostly loaded into the positively charged tube's lumen. Typical tube loading with proteins was 6-7 wt % from which one-third was released in 5-10 h and the other two-thirds remained, providing enhanced biocatalysis in nanoconfined conditions. Immobilized lipase showed enhanced stability at acidic pH, and the optimum pH shifted to more alkaline pH. Immobilized laccase was more stable with respect to time, and immobilized glucose oxidase showed retention of enzymatic activity up to 70 °C, whereas the native sample was inactive.