Analysis of the mouse protamine 1 promoter in transgenic mice.
Analysis of the mouse protamine 1 promoter in transgenic mice.
复制标题
转基因小鼠中小鼠鱼精蛋白 1 启动子的分析。
DOI：
10.1073/pnas.90.11.5071
复制
发表时间：
1993
影响因子：
11.1
通讯作者：
Palmiter,RD
中科院分区：
文献类型：
--
作者：
Zambrowicz,BP;Harendza,CJ;Zimmermann,JW;Brinster,RL;Palmiter,RD
精氨酸是一种小的富含精氨酸的蛋白质，在精子中包裹着DNA。小鼠精蛋白1 （Prm-1）基因仅在减数分裂后的精子中转录。为了鉴定精子特异性转录所需的Prm-1启动子中的元件，我们通过微量注射含有Prm-1 5‘侧翼序列的转基因小鼠，这些序列与标记的Prm-1基因相关的保守序列有5’截断或内部缺失。我们还用异源人生长激素报告基因测试了Prm-1启动子区域。我们得出结论，一个113-bp的区域可以指导精子特异性转录，并且我们已经确定了该区域内的序列，这些序列对正常功能至关重要。这些结果将有助于分离和鉴定减数分裂后基因表达所必需的转录因子。
Protamines are small arginine-rich proteins that package DNA in spermatozoa. The mouse protamine 1 (Prm-1) gene is transcribed exclusively in post-meiotic spermatids. To identify elements in the Prm-1 promoter required for spermatid-specific transcription, we generated transgenic mice by microinjection of transgenes containing Prm-1 5' flanking sequences with 5' truncations or internal deletions of conserved sequences linked to a marked Prm-1 gene. We also tested Prm-1 promoter regions with a heterologous human growth hormone reporter gene. We conclude that a 113-bp region can direct spermatid-specific transcription and we have defined sequences within this region that are essential for proper function. These results will facilitate the isolation and characterization of transcription factors essential for post-meiotic gene expression.