A Modified Technique for Heterotopic Heart Transplantation in Rats
A Modified Technique for Heterotopic Heart Transplantation in Rats
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DOI：
10.1016/j.jss.2009.05.024
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发表时间：
2010-11-01
影响因子：
2.2
通讯作者：
Li, Youping
中科院分区：
文献类型：
--
作者：
Shan, Juan;Huang, Yuchuan;Li, Youping
背景自从Ono和Lindsey发表了他们的技术以来，大鼠异位心脏移植已被广泛用于各种实验[2]。已经报道了许多改进以改进该动物模型。在这项研究中，我们描述了一种新的修改Ono和Lindsey技术。BN和刘易斯大鼠，体重200 - 250 g，用作供体和受体。将供体心脏移植到受体腹腔内，使供体头臂动脉干（BT）和右肺动脉（RPA）与受体腹动脉（AA）和下腔静脉（IVC）吻合。通过触诊和组织病理学检查评估移植物功能;定量PCR检测移植物内细胞因子mRNA表达;比较改良方法和传统Ono和Lindsey方法的血流动力学指标。在我们的改良技术中进行了30例心脏移植，其中3例移植物丢失。整个操作可在1小时内完成。组织病理学和定量PCR结果均证实存在急性移植排斥反应。此外，改良术式受者的血流动力学指标较传统术式更接近正常生理状态。我们的改良技术是一种容易学习和复制的程序，允许受体手术压力小，手术时间短，并导致高移植物和受体存活率。皇冠版权所有（C）2010由爱思唯尔公司出版。All rights reserved.
Background. Heterotopic heart transplantation in rats has been widely used in various experiments since Ono and Lindsey published their technique [2]. Many modifications have been reported to improve this animal model. In this study, we described a new modified Ono and Lindsey technique.Methods. BN and Lewis rats weighing 200 to 250g were used as donors and recipients. Donor heart was transplanted into the recipients' abdominal cavity, anastomosing the donor bracheocephalic trunk (BT) and the right pulmonary artery (RPA) to the recipient's abdominal artery (AA) and inferior vena cava (IVC). Graft function was evaluated by pulse palpation and histopathologic examination; intragraft cytokine mRNA expression was detected by quantitative PCR; the hemodynamics indexes of the recipients receiving our modified approach and traditional Ono and Lindsey technique were compared.Results. Thirty heart transplantations were carried out in our modified technique with three graft losses. The total operation can be finished within 1h. Both histopathologic findings and quantitative PCR results confirmed the presence of acute graft rejection. In addition, the hemodynamics indexes of recipients receiving our modified approach were nearer normal physiologic situation than those of recipients receiving traditional technique.Conclusion. Our modified technique is an easily learned and reproduced procedure that allows less recipient surgical stress, a shorter operation time, and leads to a high graft and recipient survival rate. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.