SEROLOGICAL DIAGNOSIS OF FELINE IMMUNODEFICIENCY VIRUS-INFECTION USING RECOMBINANT TRANSMEMBRANE GLYCOPROTEIN
SEROLOGICAL DIAGNOSIS OF FELINE IMMUNODEFICIENCY VIRUS-INFECTION USING RECOMBINANT TRANSMEMBRANE GLYCOPROTEIN
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DOI：
10.1016/0165-2427(94)07008-u
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发表时间：
1995-05-01
影响因子：
1.8
通讯作者：
LUTZ, H
中科院分区：
文献类型：
--
作者：
CALZOLARI, M;YOUNG, E;LUTZ, H
利用大肠杆菌中表达的猫免疫缺陷病毒（FIV）的重组表面（SU）、跨膜（TM）和衣壳（CA）抗原，建立了一种抗体检测酶联免疫吸附试验（ELISA）。用从实验感染的猫身上收集的血清检测这三种抗原，以便跟踪整个感染过程中的血清转化过程和抗体水平。观察到早期明显的TM抗体增加。在整个观察期间，对TM的抗体水平都很高。对SU和CA的免疫反应不太明显，在一些猫中，SU和CA的抗体水平在感染后6个月趋于下降。此外，对413例FIV阴性和阳性猫血清进行检测，以确定每种抗原的诊断敏感性、特异性和效率。TM的诊断敏感性最高（98%），特异性为97%。其诊断效率为97%，优于SU和CA，并超过了使用常规生长和梯度纯化的FIV的测试。因此，重组TM可以被认为是FIV ELISA检测中非常重要的抗原。一个有趣的观点是提供了结合TM与其他重组抗原在点化验形式。
We developed an antibody detection enzyme-linked immunosorbent assay (ELISA) using recombinant surface (SU), transmembrane (TM) and capsid (CA) antigens of feline immunodeficiency virus (FIV) expressed in Escherichia coli. The three antigens were tested with sera collected from experimentally infected cats in order to follow the course of seroconversion and of the antibody levels throughout the infection. An early and marked increase of TM antibodies was observed. Antibodies to TM were demonstrated at high levels througout the observation period. The immune response to SU and to CA was less pronounced and in some cats the level of antibodies to SU and CA tended to decline 6 months after infection.In addition, 413 FIV negative and positive cat sera were tested in order to define for each antigen the diagnostic sensitivity, specificity and efficiency. TM showed the highest diagnostic sensitivity (98%) while its specificity was 97%. Its diagnostic efficiency of 97% was better than that of SU and CA and exceeded that of tests utilizing conventionally grown and gradient purified FIV.Therefore, recombinant TM can be considered a very important antigen for FIV ELISA testing. An interesting perspective is offered in the combination of TM with other recombinant antigens in a dot assay form.