Electrochemical Resistive-Pulse Sensing
Electrochemical Resistive-Pulse Sensing
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DOI：
10.1021/jacs.9b10329
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发表时间：
2019-12-18
影响因子：
15
通讯作者：
Mirkin, Michael V.
中科院分区：
文献类型：
--
作者：
Pan, Rongrong;Hu, Keke;Mirkin, Michael V.
利用生物或固态纳米孔和纳米移液管的电阻脉冲传感已被广泛用于检测单分子和纳米颗粒。这种实验中的分析信号是由分子/颗粒移位通过移液管孔引起的离子电流的变化。本文介绍了一种新版本的连续脉冲技术的基础上使用碳纳米移液管（CNP）。所测量的电流是由碳表面的氧化还原分子的电化学氧化/还原产生的，并且响应于颗粒移位。除了对单个实体进行计数外，该技术还可以对它们所包含的电活性材料进行定性和定量分析。使用脂质体作为模型系统，我们证明了CNP用于（1）单个脂质体的常规连续脉冲传感，（2）电化学连续脉冲传感，和（3）氧化还原物质（例如，亚铁氰化物、多巴胺和亚硝酸盐）。CNP的小物理尺寸表明在生物系统中进行单实体测量的可能性。
Resistive-pulse sensing with biological or solid-state nanopores and nanopipettes has been widely employed in detecting single molecules and nanoparticles. The analytical signal in such experiments is the change in ionic current caused by the molecule/particle translocation through the pipet orifice. This paper describes a new version of the resistive-pulse technique based on the use of carbon nanopipettes (CNP). The measured current is produced by electrochemical oxidation/reduction of redox molecules at the carbon surface and responds to the particle translocation. In addition to counting single entities, this technique enables qualitative and quantitative analysis of the electroactive material they contain. Using liposomes as a model system, we demonstrate the capacity of CNPs for (1) conventional resistive-pulse sensing of single liposomes, (2) electrochemical resistive-pulse sensing, and (3) electrochemical identification and quantitation of redox species (e.g., ferrocyanide, dopamine, and nitrite) contained in a single liposome. The small physical size of a CNP suggests the possibility of single-entity measurements in biological systems.