Fabrication of tubular tissue constructs by centrifugal casting of cells suspended in an in situ crosslinkable hyaluronan-gelatin hydrogel
Fabrication of tubular tissue constructs by centrifugal casting of cells suspended in an in situ crosslinkable hyaluronan-gelatin hydrogel
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DOI：
10.1016/j.biomaterials.2005.05.061
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发表时间：
2005-12-01
期刊：
影响因子：
14
通讯作者：
Prestwich, GD
中科院分区：
文献类型：
--
作者：
Mironov, V;Kasyanov, V;Prestwich, GD
在支架中实现最佳的细胞密度和所需的细胞分布是组织工程中细胞接种技术的主要目标。为了实现这一目标，我们利用原位可交联透明质酸 (HA) 合成细胞外基质 (sECM) 开发了一种新型离心铸造技术。将活细胞悬浮在硫醇修饰的HA和硫醇修饰的明胶的粘稠溶液中，添加聚乙二醇二丙烯酸酯交联剂，在旋转过程中形成水凝胶。由致密细胞层组成的管状组织构建体是用旋转装置以 2000 rpm 运行 10 分钟来制造的。旋转前悬浮在 HA 混合物中的大多数细胞在离心铸造后位于层内。细胞在所采用的旋转方式下经历的离心力的作用下存活下来。体积细胞密度（65.6%）接近基于理论球堆积模型的最大可能体积密度。因此，离心铸造允许制造具有所需的细胞层重新分布、组成和厚度的管状结构，从而最大程度地有效利用可用细胞。这种 sECM 中的离心铸造将能够快速制造组织工程血管移植物以及其他管状和平面组织工程结构。 (c) 2005 Elsevier Ltd. 保留所有权利。
Achieving the optimal cell density and desired cell distribution in scaffolds is a major goal of cell seeding technologies in tissue engineering. In order to reach this goal, a novel centrifugal casting technology was developed using in situ crosslinkable hyaluronan-based (HA) synthetic extracellular matrix (sECM). Living cells were suspended in a viscous solution of thiol-modified HA and thiol-modified gelatin, a polyethyleneglycol diacrylate crosslinker was added, and a hydrogel was formed during rotation. The tubular tissue constructs consisting of a densely packed cell layer were fabricated with the rotation device operating at 2000 rpm for 10 min. The majority of cells suspended in the HA mixture before rotation were located inside the layer after centrifugal casting. Cells survived the effect of the centrifugal forces experienced under the rotational regime employed. The volume cell density (65.6%) approached the maximal possible volume density based on theoretical sphere packing models. Thus, centrifugal casting allows the fabrication of tubular constructs with the desired redistribution, composition and thickness of cell layers that makes the maximum efficient use of available cells. Centrifugal casting in this sECM would enable rapid fabrication of tissue-engineered vascular grafts, as well as other tubular and planar tissue-engineered constructs. (c) 2005 Elsevier Ltd. All rights reserved.