Unique poly(dA).poly(dT) B'-conformation in cellular and synthetic DNAs.
Unique poly(dA).poly(dT) B'-conformation in cellular and synthetic DNAs.
复制标题
细胞和合成 DNA 中独特的聚 (dA).聚 (dT) B 构象。
DOI：
10.1093/nar/15.15.6063
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发表时间：
1987
影响因子：
14.9
通讯作者：
Zarling,DA
中科院分区：
文献类型：
--
作者：
Diekmann,S;Zarling,DA
Poly(dA).poly(dT), but not B-form DNA, is specifically recognized by experimentally induced anti-kinetoplast or anti-poly(dA).poly(dT) immunoglobulins. Antibody binding is completely competed by poly(dA).poly(dT) and poly(dA).poly(dU) but not by other single- or double-stranded DNA sequences in a right-handed B-form. Antibody interaction with poly(dA).poly(dT) depends on immunoglobulin concentration, incubation time and temperature, and is sensitive to elevated ionic strengths. Similar conformations, for example, (dA)μ_6.(dT)μ_6, in the kinetoplast DNA of the parasiteLeishmania tarentolaeare also immunogenic and induce specific anti-poly(dA).poly(dT) antibodies. These antibody probes specifically recognize nuclear and kinetoplast DNA in fixed flagellated kinetoplastid cells as evidenced by immunofluorescence microscopy. Anti-poly(dA).poly(dT) immunofluorescence is DNase-sensitive and competed by poly(dA).poly(dT), but not other classical double-stranded B-DNAs. Thus, these unique cellular B'-DNA helices are immunogenic and structurally similar to synthetic poly(dA).poly(dT) helices in solution.
