CREB regulates MHC class II expression in a CIITA-dependent manner
CREB regulates MHC class II expression in a CIITA-dependent manner
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DOI：
10.1016/s1074-7613(00)80015-1
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发表时间：
1999-02-01
期刊：
影响因子：
32.4
通讯作者：
Boss, JM
中科院分区：
文献类型：
--
作者：
Moreno, CS;Beresford, GW;Boss, JM
MHC II类启动子的X2盒与TRE/CRE元件同源，并且是MHC II类基因表达所需的。将X2盒特异性DNA结合活性X2 BP纯化至均一，测序并鉴定为CREB。瞬时转录激活实验表明，CREB可以协同CIITA以剂量依赖性方式增强从MHC II类启动子的转录激活。通过染色质免疫沉淀试验证明CREB与II类启动子在体内的结合。此外，ICER，CREB功能的主要抑制剂，被发现抑制II类表达。这些结果表明CREB在体内结合X2盒，并与CIITA合作指导MHC II类表达。
The X2 box of MHC class II promoters is homologous to TRE/CRE elements and is required for expression of MHC class II genes. The X2 box-specific DNA binding activity, X2BP, was purified to homogeneity, sequenced, and identified as CREB. Transient transactivation experiments showed that CREB can cooperate with CIITA to enhance activation of transcription from MHC class II promoters in a dose-dependent manner. Binding of CREB to the class II promoter in vivo was demonstrated by a chromatin immunoprecipitation assay. Additionally, ICER, a dominant inhibitor of CREB function, was found to repress class II expression. These results demonstrate that CREB binds to the X2 box in vivo and cooperates with CIITA to direct MHC class II expression.