Downregulation of Na(+)-NQR complex is essential for Vibrio alginolyticus in resistance to balofloxacin
Downregulation of Na(+)-NQR complex is essential for Vibrio alginolyticus in resistance to balofloxacin
复制标题
Na()-NQR复合物的下调对于溶藻弧菌对巴洛沙星的耐药性至关重要
DOI：
10.1016/j.jprot.2012.03.006
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发表时间：
2012-05-17
影响因子：
3.3
通讯作者：
Peng, Xuan-Xian
中科院分区：
文献类型：
--
作者：
Li, Peipei;Liu, Xianjie;Peng, Xuan-Xian
据报道，在临床和水产养殖中越来越多地分离出耐药的溶藻弧菌菌株，但溶藻弧菌的耐药机制在很大程度上是未知的。本研究利用基于天然/SDS-PAGE的蛋白质组学方法，研究了溶藻弧菌对巴氟沙星耐药的差异蛋白，该方法可能提供蛋白质与蛋白质相互作用的信息。10个蛋白发生改变，其中V12G01_04671、V12G01_00457、V12G01_15927、V12G01_15240、NqrA（斑点26）、NqrF（斑点30）下调，V12G01_22043、TolC、V12G01_15130、V12G01_19297上调。重要的是，Na(+) NQR复合物的两个组分NqrA和NqrF被垂直排列，并被进一步研究。Western blotting分析表明，这两种蛋白的下调与对照蛋白TolC的上调形成鲜明对比，这与2-DE凝胶分析的结果一致。此外，NqrA、NqrF和TolC的过表达分别导致细菌在含巴氟沙星培养基中的存活能力降低和升高。这些结果表明Na(+) NQR复合物的下调是溶藻弧菌对巴氟沙星耐药的必要条件。这是首次报道Na(+) NQR复合物在抗生素耐药性中的作用。这一发现强调了理解代谢调节内容中抗生素耐药机制的方法。(C) 2012 Elsevier B.V.版权所有
Increasingly isolated frequency of antibiotic-resistant V. alginolyticus strains in clinic and aquaculture has been reported, but the mechanisms of V. alginolyticus antibiotic resistance are largely absent. In the present study, native/SDS-PAGE based proteomics, which may provide information on protein protein interaction, was utilized to investigate differential proteins of V. alginolyticus in resistance to balofloxacin. Ten proteins were altered, in which V12G01_04671, V12G01_00457, V12G01_15927, V12G01_15240, NqrA (spot 26), and NqrF (spot 30) were downregulated, while V12G01_22043, TolC, V12G01_15130, V12G01_19297 were upregulated. Importantly, the two components of Na(+) NQR complex, NqrA and NqrF, were vertically lined and was further investigated. Western blotting assay indicated that downregulation of the two proteins contrasted sharply with upregulation of a control protein TolC, which was consistent with the result obtained from 2-DE gel analysis. Furthermore, overexpression of NqrA, NqrF and TolC resulted in decrease and elevation of bacterial survival ability in medium with balofloxacin, respectively. These results indicate that downregulation of Na(+) NQR complex is essential for V. alginolyticus resistance to balofloxacin. This is the first report on the role of Na(+) NQR complex in antibiotic resistance. This finding highlights the way to an understanding of antibiotic-resistant mechanisms in content of metabolic regulation. (C) 2012 Elsevier B.V. All rights reserved.