Experimental rabies virus infection of big brown bats (Eeptesicus fuscus)
Experimental rabies virus infection of big brown bats (Eeptesicus fuscus)
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DOI：
10.7589/0090-3558-44.3.612
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发表时间：
2008-07-01
影响因子：
1.3
通讯作者：
Rupprecht, Charles E.
中科院分区：
文献类型：
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作者：
Jackson, Felix R.;Turmelle, Amy S.;Rupprecht, Charles E.
从自然感染的大棕蝠的唾液腺中分离出狂犬病病毒(RABV)变异株，并通过小鼠神经母细胞瘤细胞培养传代，对圈养的成年大棕蝠(Eptesicus Fuscus)群体进行了实验感染。蝙蝠被分成11组，每组由一到三个未感染的个体和一到三个感染的个体组成。38只动物中的20只被肌肉感染到左右咬肌；它们总共接受了10(3.2)只大棕色蝙蝠RABV变种的小鼠脑内致死量(MICLD50)。病毒暴露后的实验结果是，蝙蝠接种后140天(PI)。在20只受感染的蝙蝠中，有16只出现临床狂犬病，潜伏期平均为24天(范围为13-52天)。三只受感染的蝙蝠从未发生血清转换，并在感染早期死亡(13天)。四只受感染的蝙蝠存活到实验结束时没有任何疾病迹象，直到第三个月PI保持可检测到的抗体滴度，在第13天和43天之间达到峰值，随后在白天下降到检测阈值以下，140天。在感染蝙蝠的临床病程中，在第13天和第15天观察到两只蝙蝠在唾液中有限地排泄病毒(
A captive colony of adult Big Brown Bats (Eptesicus fuscus) was experimentally infected with a rabies virus (RABV) variant isolated from the salivary glands of a naturally infected Big Brown Bat and passaged once through murine neuroblastoma cell culture. Bats were divided into into 11 groups, which were composed of one to three noninfected and one to three infected individuals each. Twenty of 38 animals were infected intramuscularly into both left and right masseter muscles; they received a total of 10(3.2) median mouse intracerebral lethal dose (MICLD50) of Big Brown Bat RABV variant. Experimental outcome after viral exposure was if) the bats for 140 days postinoculation (PI). Of 20 infected bats, 16 developed clinical rabies, and the mean incubation period was 24 days (range: 13-52 days). Three infected bats never seroconverted and succumbed early to infection (13 days). Four infected bats that survived until the end of the experiment without any signs of disease maintained detectable antibody titers until the third month PI, peaking between days 13 and 43, and consequent drop-off below the threshold for detection occured by day, 140. Limited excretion of virus in saliva of infected bats during the clinical course of disease was observed ill two individuals on days 13 and 15 PI (