Novel components of germline sex determination acting downstream of foxl3 in medaka
Novel components of germline sex determination acting downstream of foxl3 in medaka
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DOI：
10.1016/j.ydbio.2018.10.019
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发表时间：
2019-01-01
影响因子：
2.7
通讯作者：
Tanaka, Minoru
中科院分区：
文献类型：
--
作者：
Kikuchi, Mariko;Nishimura, Toshiya;Tanaka, Minoru
生殖系的性别决定是产生两性异形配子的重要过程。在青鳉中，叉头盒L3（foxl 3）以前被确定为生殖细胞的内在调节性别决定，抑制在雌性生殖细胞精子发生的启动。为了揭示foxl 3的生殖系性别决定的分子机制，我们进行了以下四项分析：野生型和foxl 3突变生殖细胞之间的转录组的比较;上位性分析; F0 XL 3结合基序的鉴定;以及使用F0 XL 3单克隆抗体的ChIP-qPCR测定。我们鉴定了作用于foxl 3下游的两个候选基因：Rec 8a和fbxo 47。已知Rec 8调节姐妹染色单体凝聚，Fbxo 47充当泛素E3连接酶。然而，这些功能与生殖细胞的性分化无关。我们的研究结果揭示了作用于foxl 3下游的新成分，为生殖细胞性别决定机制提供了新的见解。
Germline sex determination is an essential process for the production of sexually dimorphic gametes. In medaka, Forkhead box L3 (foxl3) was previously identified as a germ cell-intrinsic regulator of sex determination that suppresses the initiation of spermatogenesis in female germ cells. To reveal the molecular mechanism of germline sex determination by foxl3, we conducted the following four analyses: Comparison of transcriptomes between wild-type and foxl3-mutant germ cells; epistatic analysis; identification of the FOXL3-binding motif; and ChIP-qPCR assay using a FOXL3-monoclonal antibody. We identified two candidate genes acting downstream of foxl3: Rec8a and fbxo47. It has been known that Rec8 regulates sister chromatid cohesion and Fbxo47 acts as a ubiquitin E3 ligase. These functions have not been, however, associated with sexual differentiation in germ cells. Our results uncover novel components acting downstream of foxl3, providing insights into the mechanism of germline sex determination.