Generating ready-to-implant anisotropic menisci by 3D-bioprinting protein-releasing cell-laden hydrogel-polymer composite scaffold
Generating ready-to-implant anisotropic menisci by 3D-bioprinting protein-releasing cell-laden hydrogel-polymer composite scaffold
复制标题
通过 3D 生物打印释放蛋白质的充满细胞的水凝胶-聚合物复合支架生成可植入的各向异性半月板
DOI：
10.1016/j.apmt.2019.100469
复制
发表时间：
2020-03-01
影响因子：
8.3
通讯作者：
Dai, Kerong
中科院分区：
文献类型：
--
作者：
Sun, Ye;You, Yongqing;Dai, Kerong
目的：三维打印生物材料支架已被报道产生不同的组织。然而，3D打印生成的支架需要进一步的细胞接种和体外长期培养。产生仿生半月板组织模仿原生meniscus的各向异性性质仍然是一个挑战。方法：我们报告通过3D生物打印蛋白质释放细胞负载的水凝胶-PCL复合支架产生集成的活半月板构建。简而言之，将PCL熔融以制造用于支架的物理支撑结构，同时将包封在不同区域中携带TGF β 3或CTGF以诱导各向异性表型的PLGA微粒的载有MSC细胞的水凝胶生物打印到来自不同注射器的PCL纤维之间的微通道中。在体外和山羊半月板移植模型中对3D生物打印半月板进行了评估。结果：再生半月板结构中的细胞表型和基质沉积与体外和体内的天然各向异性半月板相似。此外，将3D生物打印的半月板移植到山羊膝关节中赋予了长期的软骨protection.Conclusion：山羊各向异性半月板构建准备立即植入并模仿天然半月板是由3D生物打印蛋白释放细胞负载水凝胶聚合物复合物支架产生的。(C)2019爱思唯尔有限公司版权所有。
Objectives: Three-dimensional-printed biomaterial scaffolds have been reported to generate different tissues. However, 3D-printing generated scaffolds require further cell seeding and long-term cultivation in vitro. Generating biomimetic meniscus tissue mimicking the anisotropic nature of the native meniscus still remains a challenge.Methods: We report generating integrated living meniscus construct by 3D-bioprinting protein-releasing cell-laden Hydrogel-PCL composite scaffold. Briefly, PCL was molten to fabricate the physically supporting structure for the scaffold while MSC cell-laden hydrogel encapsulating PLGA microparticles carrying TGF beta 3 or CTGF in different regions to induce anisotropic phenotypes were bio-printed into the microchannels between PCL fibers from different syringes. The 3D-bioprinted meniscus was evaluated in vitro and in a goat meniscus transplantation model.Results: Cell phenotypes and matrix deposition in the regenerated meniscus construct showed resemblance to the native anisotropic meniscus in vitro and in vivo. Moreover, Transplantation of the 3D-bioprinted meniscus into goat knees conferred long-term chondroprotection.Conclusion: Goat anisotropic meniscus construct ready for immediate implantation and mimicking the native meniscus was generated by 3D-Bioprinting Protein-releasing Cell-laden Hydrogel-Polymer composite Scaffold. (C) 2019 Elsevier Ltd. All rights reserved.