Do all anti-citrullinated protein/peptide antibody tests measure the same? Evaluation of discrepancy between anti-citrullinated protein/peptide antibody tests in patients with and without rheumatoid arthritis
Do all anti-citrullinated protein/peptide antibody tests measure the same? Evaluation of discrepancy between anti-citrullinated protein/peptide antibody tests in patients with and without rheumatoid arthritis
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DOI：
10.1136/ard.2007.071654
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发表时间：
2008-04-01
影响因子：
27.4
通讯作者：
De Keyser, F.
中科院分区：
文献类型：
--
作者：
Cruyssen, B. Vander;Nogueira, L.;De Keyser, F.
背景资料：不同的方法存在，以证明抗瓜氨酸蛋白/肽抗体（ACPA）aims：为了评估四个ACPA tests.Patients和方法之间的差异：人口1包括一个新的诊断问题的患者，包括86例类风湿关节炎（RA）和450例无RA患者。人群2包括155例长期患病的RA患者。人群3包括188例银屑病关节炎患者，人群4包括192例系统性红斑狼疮患者。人群1和2采用抗人纤维蛋白原抗体（AhcA）检测、Eurodiagnosis公司的抗CCP 2（CCP 2-euro）、Pharmacia公司的抗CCP 2（CCP 2-phar）和Inova公司的抗CCP 3检测（CCP 3）进行检测。如果样本在一项检测中呈阳性，而在至少一项其他检测中呈阴性，则将样本注释为不一致。在不同的运行中重新分析每个不一致样品。人群3和4进行了分析，在CCP 2-欧元和AhFibA test.Results：在人群1中，ACPA阳性被发现在17 450例（3.8%）没有RA，14（82%）这17个样本是不一致的。相反，86例RA患者中有61例（70.9%）ACPA阳性，其中18例（29.5%）ACPA阳性不一致（70.9%对29.5%，p
Background: Different methods exist to demonstrate anti-citrullinated protein/peptide antibodies (ACPA).Aims: To evaluate discrepancy between four ACPA tests.Patients and methods: Population 1 consisted of patients with a new diagnostic problem, including 86 patients with rheumatoid arthritis (RA) and 450 patients without RA. Population 2 consisted of 155 patients with RA who had long-standing disease. Population 3 consisted of 188 patients with psoriatic arthritis and in population 4 there were 192 patients with systemic lupus erythematosus. Populations 1 and 2 were tested with the anti-human fibrinogen antibody (AhfibA) test, anti-CCP2 from Eurodiagnostica (CCP2-euro), anti-CCP2 from Pharmacia (CCP2-phar) and anti-CCP3 test by Inova (CCP3). Samples were annotated as discrepant if positive in one and negative in at least one other test. Each discrepant sample was re-analysed in a different run. Populations 3 and 4 were analysed in the CCP2-euro and AhFibA test.Results: In population 1, ACPA positivity was found in 17 of 450 (3.8%) patients without RA; 14 (82%) of these 17 samples were discrepant. In contrast, 61 of 86 (70.9%) patients with RA were ACPA positive of whom 18 of 61 (29.5%) were discrepant (70.9% vs. 29.5%, p