The effect of solvent on permeant diffusion through membranes studied using ATR-FTIR and chemometric data analysis
The effect of solvent on permeant diffusion through membranes studied using ATR-FTIR and chemometric data analysis
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DOI：
10.1002/jps.10530
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发表时间：
2004-01-01
影响因子：
3.8
通讯作者：
Tetteh, J
中科院分区：
文献类型：
--
作者：
Dias, M;Hadgraft, J;Tetteh, J
提高局部制剂的生物利用度的一种方法是加入适当的溶剂，其将充当渗透物的增溶剂，同时改变角质层的屏障性质。已证明很难确定所涉及的确切作用机制;这是复杂的同时扩散的溶剂和渗透到皮肤。在这种情况下，屏障功能很可能会随着时间的变化而变化，因为溶剂会破坏它。我们在一个模型硅膜系统中观察到这种现象，我们选择最初研究该系统，以避免皮肤异质性的复杂性及其固有的生物变异性。扩散实验进行了使用一个既定的ATR-FTIR方法，但使用复杂的化学计量学方法，使我们能够去卷积的渗透，溶剂和膜的IR信号解释的数据。所提供的数据显示了苯甲酸（渗透物）、辛醇（溶剂）的同时扩散，以及辛醇如何改变硅树脂膜的特性。初始数据，然后使用人类皮肤显示的权力的扩散方法耦合到数据反卷积技术。(C)2004威利-利斯公司
One method of improving the bioavailability of a topical formulation is to add an appropriate solvent that will act as a solubilizer for the permeant and, at the same time, modify the barrier properties of the stratum corneum. It has proved very difficult to determine the precise mechanisms of action involved; this is complicated by the concurrent diffusion of the solvent and the permeant into the skin. Under these circumstances the barrier function may well be changing as a function of time as the solvent disrupts it. We have observed this phenomenon in a model silicone membrane system that we have chosen to study initially to avoid the complexity of the heterogeneous nature of skin and its inherent biological variability. Diffusion experiments were conducted using an established ATR-FTIR approach but the data interpreted using sophisticated chemometric approaches that allowed us to deconvolve the IR signals from the permeant, the solvent, and the membrane. Data are presented that show the concurrent diffusion of benzoic acid (permeant), octanol (solvent), and how the octanol modifies the characteristics of the silicone membrane. Initial data are then presented using human skin to show the power of the diffusion approach coupled to the data deconvolution technique. (C) 2004 Wiley-Liss, Inc.