New technique to take samples from environmental surfaces using flocked nylon swabs
New technique to take samples from environmental surfaces using flocked nylon swabs
复制标题
DOI：
10.1016/j.jhin.2010.02.027
复制
发表时间：
2010-08-01
影响因子：
6.9
通讯作者：
Lore, B.
中科院分区：
文献类型：
--
作者：
Hedin, G.;Rynback, J.;Lore, B.
医院中受感染和/或定植患者附近的环境表面通常被潜在致病微生物污染。然而，目前还没有标准化的方法从表面取样以进行定量培养。通常使用接触板或拭子，但这些方法可能会给出不同的结果。传统抽汲的采收率，e。G.棉花或人造丝，都很差。与人造丝拭子相比，使用植绒尼龙的新型拭子的回收率可提高三倍。在这项研究中，我们将金黄色葡萄球菌和肠球菌的参考菌株接种到床头柜上，并在1小时后当接种物干燥时取样。从同一表面上连续取样。对每个样品使用两个连续尼龙拭子的新采样技术进行了验证。的采样效率，接种物的回收率和从重复实验中获得的培养结果的变化进行了描述。使用两个连续植绒尼龙拭子进行采样，证明了接种物的效率提高和回收率提高。(C)2010年医院感染学会。由爱思唯尔有限公司出版。保留所有权利。
Environmental surfaces near infected and/or colonised patients in hospitals are commonly contaminated with potentially pathogenic micro-organisms. At present, however, there is no standardised method for taking samples from surfaces in order to perform quantitative cultures. Usually contact plates or swabs are used, but these methods may give different results. The recovery rate of traditional swabbing, e. g. cotton or rayon, is poor. With a new type of swab utilising flocked nylon, the recovery may be enhanced up to three times compared with a rayon swab. In this study, we inoculated reference strains of Staphylococcus aureus and Enterococcus hirae onto a bedside table and took samples 1 h later when inocula were dry. Sequential samples were taken from the same surface. A new sampling technique using two sequential nylon swabs for each sample was validated. The efficiency of the sampling, percentage recovery of the inoculum and the variation of culture results obtained from repeated experiments are described. Enhanced efficiency and higher recovery of inoculum were demonstrated using two sequential flocked nylon swabs for sampling. (C) 2010 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.