PRC1: A human mitotic spindle-associated CDK substrate protein required for cytokinesis
PRC1: A human mitotic spindle-associated CDK substrate protein required for cytokinesis
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DOI：
10.1016/s1097-2765(00)80302-0
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发表时间：
1998-12-01
期刊：
影响因子：
16
通讯作者：
Fukunaga, R
中科院分区：
文献类型：
--
作者：
Jiang, W;Jimenez, G;Fukunaga, R
我们已经鉴定出一种新的人类蛋白 PRC1，它参与胞质分裂。 PRC1在体外是多种CDK的刺激底物，并且在体内在体外被CDK磷酸化的位点被磷酸化，强烈表明PRC1是体内CDK底物，PRC1与出芽酵母后期纺锤体伸长因子Ase1p具有序列同源性。与 Ase1p 一样，PRC1 蛋白水平在 S 和 G2/M 期间很高，但在细胞退出有丝分裂并进入 G1 后急剧下降。 PRC1是间期的核蛋白，在有丝分裂期间以高度动态的方式与有丝分裂纺锤体相关，在胞质分裂期间最终定位于细胞中间体。将抗 PRC1 抗体显微注射到 HeLa 细胞中会阻止细胞分裂，但不会阻止核分裂，这表明 PRC1 在胞质分裂过程中发挥功能作用。
We have identified a novel human protein, PRC1, that is involved in cytokinesis. PRC1 is a goad substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate, PRC1 has sequence homology to the budding yeast anaphase spindle Elongation factor Ase1p. Like Ase1p, PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit: mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, end localizes to the cell midbody during cytokinesis. Microinjection of anti-PRC1 antibodies into HeLa cells blocked cellular cleavage, hut not nuclear division, indicating a functional role for PRC1 in the process of cytokinesis.