SulfoSYS (Sulfolobus Systems Biology): towards a silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
SulfoSYS (Sulfolobus Systems Biology): towards a silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
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DOI：
10.1042/bst0370058
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发表时间：
2009-02-01
影响因子：
3.9
通讯作者：
Zaparty, Melanie
中科院分区：
文献类型：
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作者：
Albers, Sonja-Verena;Birkeland, Nils-Kare;Zaparty, Melanie
SulfoSYS（Sulfolobus Systems Biology）重点研究硫磺叶菌的CCM（中央碳水化合物代谢）及其在系统水平上温度变化下的调控。在古细菌中，碳水化合物是通过改变细菌或真核生物已知的经典途径来代谢的。不寻常的分支 ED（Entner-Doudoroff）途径，用于 S. solfataricus 中的葡萄糖降解。选择的这种古菌模型生物是一种嗜热嗜酸的骨裂菌，其最佳生长温度为 80 摄氏度（60-92 摄氏度）和 pH 2-4。一般来说，高温下的生命需要非常有效地适应温度变化，这对生物体来说是最难应对的，目前还不清楚生物网络如何承受和响应这种变化。这个综合项目结合了基因组学、转录组学、蛋白质组学和代谢组学以及动力学和生化信息。 SulfoSYS 的最终目标是为活细胞的这一部分构建硅细胞模型，从而实现 CCM 网络的计算。在本文中，我们报告了第一个古菌系统生物学项目。
SulfoSYS (Sulfolobus Systems Biology) focuses on the study of the CCM (central carbohydrate metabolism) of Sulfolobus solfataricus and its regulation under temperature variation at the systems level. in Archaea, carbohydrates are metabolized by modifications of the classical pathways known from Bacteria or Eukarya, e.g. the unusual branched ED (Entner-Doudoroff) pathway, which is utilized for glucose degradation in S. solfataricus. This archaeal model organism of choice is a thermoacidophilic crenarchaeon that optimally grows at 80 degrees C (60-92 degrees C) and pH 2-4. In general, life at high temperature requires very efficient adaptation to temperature changes, which is most difficult to deal with for organisms, and it is unclear how biological networks can withstand and respond to such changes. This integrative project combines genomic, transcriptomic, proteomic and metabolomic, as well as kinetic and biochemical information. The final goal of SulfoSYS is the construction of a silicon cell model for this part of the living cell that will enable computation of the CCM network. In the present paper, we report on one of the first archaeal systems biology projects.