The effects of food restriction and hypophysectomy on numbers of primordial follicles and concentrations of hormones in rats.
The effects of food restriction and hypophysectomy on numbers of primordial follicles and concentrations of hormones in rats.
复制标题
食物限制和垂体切除术对大鼠原始卵泡数量和激素浓度的影响。
DOI：
10.1095/biolreprod35.1.68
复制
发表时间：
1986
影响因子：
3.6
通讯作者：
Butcher,RL
中科院分区：
文献类型：
--
作者：
Meredith,S;Kirkpatrick-Keller,D;Butcher,RL
Three experiments were done to examine the effects of food restriction, beginning at 21 days of age, on loss of primordial follicles and on concentrations of gonadotropins and sex steroids in rats. In Experiment 1, food restriction (FR) from 21 to 51–55 days of age had no effect on number of primordial follicles, but increased the plasma concentration (p<0.05) of follicle stimulating hormone (FSH). (p<0.05). In Experiment 2, comparisons were made of groups of rats (1) fed ad libitum (AL) (2) hypophysectomized at 21 days of age and fed ad libitum (AL-HY), (3) food restriction from 21 to 52–58 days of age (FR), and (4) food restriction with twice-daily injections of follicular fluid (FR-FF). Hypophysectomy was the only treatment that decreased the loss of primordial follicles (p<0.001). Concentrations of FSH were decreased in AL-HY and increased in FR and FR-FF rats (144 ± 13, 53 ± 15, 275 ± 30 and 359 ± 56 ng/ml in AL, AL-HY, FR and FR-FF rats, respectively). Concentrations of luteinizing hormone (LH) were lower (p<0.05) in AL-HY, FR and FR-FF rats than in AL rats. In Experiment 3, AL and FR rats were unilaterally ovariectomized (ULO) at 30 days of age. Blood samples were taken 5 days prior to ULO, at ULO and at 12 h, 5 days, and 22–28 days after ULO. Concentrations of FSH increased (p<0.05) in FR rats between 25 and 30 days of age and remained elevated throughout the experimental period with no additional increase after ULO. Concentrations of FSH in AL rats increased (p<0.001) 12 h after ULO, then returned to a basal level similar to metestrus. Puberty was delayed in all FR rats. In hypophysectomized rats, concentrations of FSH were low and the rate of loss of primordial follicles was slowed. In contrast, FR rats had elevated levels of FSH and no decrease in loss of oocytes.
