Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae)
Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae)
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DOI：
10.1016/s0168-9452(00)00367-8
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发表时间：
2000-12-07
期刊：
影响因子：
5.2
通讯作者：
Chang, WC
中科院分区：
文献类型：
--
作者：
Chen, JT;Chang, WC
以合轴兰杂种文心兰为材料，建立了一种高效的体细胞胚胎发生和植株再生的方法。在1/2 MS [11]基本培养基上，由茎和叶段的根尖和切端形成致密的黄白色胚性愈伤组织，该培养基补充有1-苯基-3-（1，2，3-噻二唑-5-基）-脲（TDZ，0.1-3 mg/l）、2，3-二氯苯氧基乙酸（2，4-D，3-10 mg/l）和蛋白胨（1 g/l），持续4-7周。胚性愈伤组织在诱导愈伤组织的同一培养基上继代培养，1个月内增殖2-4倍（鲜重）。在无蔗糖的基本培养基上，诱导体细胞胚发生，并从愈伤组织培养物中分化出类原球茎（PLBs）。再生体从体细胞胚（SE）转移到相同的培养基后回收，并表现出正常的发展。从愈伤组织诱导到成苗需要12-14周。根源愈伤组织的成胚率高于茎、叶源愈伤组织。萘乙酸（NAA）和TDZ的组合显着促进胚性愈伤组织培养。在附加0.1 mg/l NAA和3 mg/l TDZ的基本培养基上，在根源愈伤组织中发现了高频率（93.8%）的体细胞胚胎发生和平均29.1 SE PET愈伤组织（3 x 3 mm（2））。盆栽水苔和温室驯化后，几乎所有的SE都转化了，试管苗生长良好，成活率接近100%。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
An efficient method was established for high frequency somatic embryogenesis and plant regeneration from callus cultures of a hybrid of sympodial orchid (Oncidium 'Gower Ramsey'). Compact and yellow-white embryogenic calli formed from root tips and cut ends of stein and leaf segments on 1/2 MS [11] basal medium supplemented with 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ, 0.1-3 mg/l), 2,3-dichlorophenoxyacetic acid (2,4-D, 3-10 mg/l) and peptone (1 g/l) for 4-7 weeks. Embryogenic callus was maintained by subculture on the same medium For callus induction and proliferated 2-4 times (fresh weight) in 1 month. Initiation of somatic embryogenesis and development up to the protocorm-like-bodies (PLBs) from callus cultures was achieved on hormone-free basal medium. Regenerants were recovered from somatic embryos (SEs) after transfer to the same medium and showed normal development. The optimized protocol required about 12-14 weeks from the initiation of callus to the plantlet formation. Generally, the frequency of embryo formation of root-derived callus was higher than stem- and leaf-derived calli. Combinations of naphthaleneacetic acid (NAA) and TDZ significantly promoted embryo formation from callus cultures. The high-frequency (93.8%) somatic embryogenesis and an average of 29.1 SEs pet callus (3 x 3 mm(2)) was found in root-derived callus on a basal medium supplemented with 0.1 mg/l NAA and 3 mg/l TDZ. Almost all the SEs converted and the plantlets grew well with an almost 100% survival rate when potted in sphagnum moss and acclimatized in the greenhouse. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.