Elucidating the origins of the vascular system: a fate map of the vascular endothelial and red blood cell lineages in Xenopus laevis.
Elucidating the origins of the vascular system: a fate map of the vascular endothelial and red blood cell lineages in Xenopus laevis.
复制标题
阐明血管系统的起源：非洲爪蟾血管内皮细胞和红细胞谱系的命运图。
DOI：
10.1006/dbio.1999.9245
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Saha,MS
中科院分区：
文献类型：
--
作者：
Mills,KR;Kruep,D;Saha,MS
血管系统是脊椎动物胚胎发育过程中发育的第一个功能器官系统，为发育中的胚胎提供营养和氧气。虽然在确定调节血管发育的遗传级联方面取得了实质性进展，但血管发生的初始阶段，即早期胚胎内血管内皮细胞的起源，仍然不清楚。为了解决这个问题，我们构建了一个特定血管结构的命运图，包括主动脉弓、内膜、背主动脉、主静脉和侧腹静脉，以及非洲爪蟾16细胞期和32细胞期的红细胞。使用遗传标记来识别这些细胞类型，我们的结果表明，血管内皮细胞实际上可以从16细胞期和32细胞期胚胎的每个卵裂球中产生，不同的卵裂球优先，但不是唯一的，从而产生特定的血管结构。类似地，但更令人惊讶的是，16细胞期胚胎中的每个卵裂球和32细胞期胚胎中除大多数动物层外的所有卵裂球都是红细胞的祖细胞。总之，我们的研究结果表明，在正常发育过程中，背侧和腹侧卵裂球都对血管内皮细胞和红细胞谱系有显着贡献。
Required to supply nutrients and oxygen to the growing embryo, the vascular system is the first functional organ system to develop during vertebrate embryogenesis. Although there has been substantial progress in identifying the genetic cascade regulating vascular development, the initial stages of vasculogenesis, namely, the origin of vascular endothelial cells within the early embryo, remain unclear. To address this issue we constructed a fate map for specific vascular structures, including the aortic arches, endocardium, dorsal aorta, cardinal veins, and lateral abdominal veins, as well as for the red blood cells at the 16-cell stage and the 32-cell stage ofXenopus laevis.Using genetic markers to identify these cell types, our results suggest that vascular endothelial cells can arise from virtually every blastomere of the 16-cell-stage and the 32-cell-stage embryo, with different blastomeres preferentially, though not exclusively, giving rise to specific vascular structures. Similarly, but more surprisingly, every blastomere in the 16-cell-stage embryo and all but those in the most animal tier of the 32-cell-stage embryo serve as progenitors for red blood cells. Taken together, our results suggest that during normal development, both dorsal and ventral blastomeres contribute significantly to the vascular endothelial and red blood cell lineages.