The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes.
The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes.
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DOI：
10.1371/journal.pone.0009471
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发表时间：
2010-03-01
期刊：
影响因子：
3.7
通讯作者：
Walter MF
中科院分区：
文献类型：
--
作者：
Biessmann H;Andronopoulou E;Biessmann MR;Douris V;Dimitratos SD;Eliopoulos E;Guerin PM;Iatrou K;Justice RW;Kröber T;Marinotti O;Tsitoura P;Woods DF;Walter MF
吸血昆虫经常是包括疟疾在内的寄生虫病的携带者。冈比亚按蚊是撒哈拉以南非洲疟疾的主要传播媒介，因此每天造成数千人死亡。虽然嗅觉在A.尽管已经证明了冈比亚宿主检测，但是关于配体和气味结合蛋白（OBP）的组合可以在体内产生特异性气味相关反应的知之甚少。我们鉴定了A的配体吲哚。冈比亚气味结合蛋白AgamOBP 1模拟了这种相互作用，并使用生物化学测定证实了这种相互作用。RNAi介导的基因沉默结合电生理分析证实AgamOBP 1在A.冈比亚和触角受体细胞不响应吲哚的AgamOBP 1的情况下。这个案例代表了第一个有记录的特定A的实例。冈比亚OBP-配体配对组合，证明了OBP在气味识别中的重要性，并且可以扩展到鉴定按蚊和其他医学重要昆虫的OBP的其他配体。
Haematophagous insects are frequently carriers of parasitic diseases, including malaria. The mosquito Anopheles gambiae is the major vector of malaria in sub-Saharan Africa and is thus responsible for thousands of deaths daily. Although the role of olfaction in A. gambiae host detection has been demonstrated, little is known about the combinations of ligands and odorant binding proteins (OBPs) that can produce specific odor-related responses in vivo. We identified a ligand, indole, for an A. gambiae odorant binding protein, AgamOBP1, modeled the interaction in silico and confirmed the interaction using biochemical assays. RNAi-mediated gene silencing coupled with electrophysiological analyses confirmed that AgamOBP1 binds indole in A. gambiae and that the antennal receptor cells do not respond to indole in the absence of AgamOBP1. This case represents the first documented instance of a specific A. gambiae OBP–ligand pairing combination, demonstrates the significance of OBPs in odor recognition, and can be expanded to the identification of other ligands for OBPs of Anopheles and other medically important insects.