DROSOPHILA SCAFFOLD-ATTACHED REGIONS BIND NUCLEAR SCAFFOLDS AND CAN FUNCTION AS ARS ELEMENTS IN BOTH BUDDING AND FISSION YEASTS
DROSOPHILA SCAFFOLD-ATTACHED REGIONS BIND NUCLEAR SCAFFOLDS AND CAN FUNCTION AS ARS ELEMENTS IN BOTH BUDDING AND FISSION YEASTS
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DOI：
10.1128/mcb.10.10.5442
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发表时间：
1990-10-01
影响因子：
5.3
通讯作者：
GASSER, SM
中科院分区：
文献类型：
--
作者：
AMATI, B;GASSER, SM
组蛋白缺失的细胞核在支架附着区（SARs）或基质附着区与基因组DNA保持序列特异性相互作用。我们之前已经证明，在酿酒酵母中，自主复制的序列元件与核支架结合。在这里，我们将这些观察结果扩展到裂糖酵母。此外，我们发现先前在黑腹果蝇基因组DNA中定位的四个SARs在体外与两种酵母的核支架结合。鉴于这些结果，我们分析了果蝇SARs在两种酵母中促进质粒自主复制的能力。其中2个在出芽酵母中具有自主复制序列活性，3个在裂变酵母中具有自主复制序列活性；而侧翼的4个非sar序列则完全失活。
Histone-depleted nuclei maintain sequence-specific interactions with genomic DNA at sites known as scaffold attachment regions (SARs) or matrix attachment regions. We have previously shown that in Saccharomyces cerevisiae, autonomously replicating sequence elements bind the nuclear scaffold. Here, we extend these observations to the fission yeast Schizosaccharomyces pombe. In addition, we shown that four SARs previously mapped in the genomic DNA of Drosophila melanogaster bind in vitro to nuclear scaffolds from both yeast species. In view of these results, we have assayed the ability of the Drosophilia SARs to promote autonomous replication of plasmids in the two yeast species. Two of the Drosophila SARs have autonomously replicating sequence activity in budding yeast, and three function in fission yeast; while four flanking non-SAR sequences are totally inactive in both.