Keratolinin: the soluble substrate of epidermal transglutaminase from human and bovine tissue.
Keratolinin: the soluble substrate of epidermal transglutaminase from human and bovine tissue.
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角蛋白：来自人和牛组织的表皮转谷氨酰胺酶的可溶性底物。
DOI：
10.1073/pnas.81.1.238
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发表时间：
1984
影响因子：
11.1
通讯作者：
Wuepper,KD
中科院分区：
文献类型：
--
作者：
Zettergren,JG;Peterson,LL;Wuepper,KD
通过离子交换色谱法和制备型区带电泳分离和纯化人和牛表皮转氨酶的底物（精氨酸-肽γ-谷氨酰转移酶，R-精氨酸-肽：胺-γ-谷氨酰转移酶，EC 2.3.2.13）。这些分子量为36，000的底物，我们建议称之为角质素，它与丹磺酰尸胺结合，并被抗体沉淀。角蛋白最终在角质细胞膜的内小叶上聚合以形成角膜包膜。每个Mr为36，000的底物被离液剂或去污剂解离成非共价亚基;在15%丙烯酰胺/1% NaDodSO 4/6 M尿素凝胶中电泳，这些亚基的Mr为6，000 - 6，200。人或牛角质素的等电聚焦显示两个部分相隔0.3-0.4 pH单位（人，5.4/5.0;牛，6.3/6.0）。这两种蛋白质很容易解决的层析聚焦和每个等电点部分的牛角质素纳入丹磺尸胺的表皮转氨酶和钙和反应的身份抗血清可溶性Mr 36,000角质素。抗人角蛋白的抗血清不能与牛角蛋白发生交叉反应。抗血清外皮蛋白没有交叉反应，无论是角蛋白或表皮免疫扩散。人和牛表皮角质素是生物化学上相似但免疫化学上不同的表皮蛋白质。在细胞培养中，外膜蛋白仅以显著量出现。
Substrates of human and bovine epidermal transglutaminase (glutaminyl-peptide gamma-glutamyltransferase, R-glutaminyl-peptide:amine-gamma-glutamyltransferase, EC 2.3.2.13) were isolated and purified by ion exchange chromatography and preparative zone electrophoresis. These substrates of Mr 36,000, which we propose to call keratolinin, incorporated dansylcadaverine and were precipitated by antibody. Keratolinin is ultimately polymerized on the inner leaflet of the keratinocyte membrane to form the cornified envelope. Each Mr 36,000 substrate was dissociated by chaotropic agents or detergents into noncovalent subunits; the Mr of these subunits was 6,000-6,200 on electrophoresis in 15% acrylamide/1% NaDodSO4/6 M urea gels. Isoelectric focusing of human or bovine keratolinin revealed two moieties separated by 0.3-0.4 pH unit (human, 5.4/5.0; bovine, 6.3/6.0). The two proteins were readily resolved by chromatofocusing and each isoelectric moiety of bovine keratolinin incorporated dansylcadaverine by epidermal transglutaminase and calcium and reacted with identity to antiserum to soluble Mr 36,000 keratolinin. Antiserum to human keratolinin failed to crossreact with its bovine counterpart. Antiserum to involucrin did not crossreact with either keratolinin or epidermis by immunodiffusion. Human and bovine epidermal keratolinins are biochemically similar but immunochemically distinct proteins from the epidermis. Involucrin appears only in significant quantities in cell culture.