Colour-barcoded magnetic microparticles for multiplexed bioassays
Colour-barcoded magnetic microparticles for multiplexed bioassays
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DOI：
10.1038/nmat2815
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发表时间：
2010-09-01
期刊：
影响因子：
41.2
通讯作者：
Kwon, Sunghoon
中科院分区：
文献类型：
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作者：
Lee, Howon;Kim, Junhoi;Kwon, Sunghoon
编码颗粒在药物发现和临床诊断等多路高通量生物分析中具有被证明的价值(1，2)。在不同的样品中，在分析颗粒上使用大量不同的识别代码的能力对于增加产量是重要的(3)。还需要适当的处理方案来读出自由漂浮探针微粒上的这些代码。在这里，我们使用一种颜色可调的磁性材料和一种新的打印方法(4)，创造出具有多轴旋转控制的生动、自由浮动的结构彩色粒子。我们的彩色条形码磁性微粒具有独特的磁性处理能力，可轻松编码数十亿个单位(5)，包括主动定位编码读数和主动搅拌以改进微尺度环境中的反应动力学。使用彩色条形码磁性微粒进行DNA杂交测试，以展示多路复用能力。
Encoded particles have a demonstrated value for multiplexed high-throughput bioassays such as drug discovery and clinical diagnostics(1,2). In diverse samples, the ability to use a large number of distinct identification codes on assay particles is important to increase throughput(3). Proper handling schemes are also needed to readout these codes on free-floating probe microparticles. Here we create vivid, free-floating structural coloured particles with multi-axis rotational control using a colour-tunable magnetic material and a new printing method(4). Our colour-barcoded magnetic microparticles offer a coding capacity easily into the billions with distinct magnetic handling capabilities including active positioning for code readouts and active stirring for improved reaction kinetics in microscale environments(5). A DNA hybridization assay is done using the colour-barcoded magnetic microparticles to demonstrate multiplexing capabilities.