Effect of sensory deafferentation on the gabaergic circuitry of the adult cat visual system
Effect of sensory deafferentation on the gabaergic circuitry of the adult cat visual system
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DOI：
10.1016/s0306-4522(97)00422-3
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发表时间：
1998-03-01
期刊：
影响因子：
3.3
通讯作者：
Vandesande, F
中科院分区：
文献类型：
--
作者：
Arckens, L;Eysel, UT;Vandesande, F
采用原位杂交技术研究了双侧视网膜中央病变对成年猫膝状背外侧核、膝状旁核和视觉皮层GAD（67）和GAD（65）信使RNA水平的影响。病变后3天，失神经传入的膝状背外侧核中表达GAD（67）的细胞数量明显减少。这种下降一直持续到病变后7.5个月，并且随着生存时间的延长而更加明显。谷氨酸脱羧酶免疫反应细胞的减少反映了GAD(67) mRNA的减少。GAD（65）信使RNA在对照组和视网膜病变猫的膝状背外侧核中的表达水平均较低。在孕周核中，两种谷氨酸脱羧酶异构体的信使RNA水平在一个限制区域内明显降低。在病变影响的视觉皮层中，GAD（67）或GAD（65）均未观察到信使RNA水平的变化，尽管先前已描述了谷氨酸脱羧酶免疫反应性的变化。（34）因此，在膝状核背外侧、膝状核周围和视觉皮层中，不同的细胞内机制似乎导致gaba能抑制减少，以响应感觉传入。(c) 1997年。Elsevier Science Ltd.出版。
The effect of bilateral central retinal lesions on the GAD(67) and GAD(65) messenger RNA levels in the dorsal lateral geniculate nucleus, the perigeniculate nucleus and the visual cortex of the adult cat was investigated by in situ hybridization. Three days post-lesion, a decrease in the number of GAD(67)-expressing cells was apparent in the deafferented dorsal lateral geniculate nucleus. This decrease persisted until 7.5 months post-lesion and was more pronounced with longer survival times. The decrease in GAD(67) mRNA was mirrored by a decrease in glutamate decarboxylase-immunoreactive cells. GAD(65) messenger RNA expression levels were low in the dorsal lateral geniculate nucleus of both control and retinally-lesioned cats. In the perigeniculate nucleus the messenger RNA levels of both glutamate decarboxylase isoforms were clearly decreased over a restricted region. In the lesion-affected visual cortex, no changes at the messenger RNA level were observed for either GAD(67) or GAD(65) although changes in glutamate decarboxylase immunoreactivity have been previously described.(34)Hence, in the dorsal lateral geniculate nucleus, the perigeniculate nucleus and the visual cortex, different intracellular mechanisms seem to lead to decreased GABAergic inhibition in response to sensory deafferentation. (C) 1997 IBRO. Published by Elsevier Science Ltd.